Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP
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DOI：
10.1016/s0896-6273(00)00084-2
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发表时间：
2000-10-01
期刊：
影响因子：
16.2
通讯作者：
Sanes, JR
中科院分区：
文献类型：
--
作者：
Feng, GP;Mellor, RH;Sanes, JR
我们产生了转基因小鼠，其中红色，绿色，黄色或青色荧光蛋白（统称为XFP）选择性地在神经元中表达。所有四种XFP都标记了整个神经元，包括轴突、神经末梢、树突和树突棘。值得注意的是，25个独立产生的转基因株系中的每一个都以独特的模式表达XFP，即使所有的转基因株系都包含相同的调控元件（来自thy1基因）。例如，在一些细胞系中，所有视网膜神经节细胞或许多皮质神经元都是XFP阳性的，而在其他细胞系中，只有少数神经节细胞或只有第5层皮质锥体被标记。在一些线路，强烈的标记小神经元亚群提供了高尔基体样的活体染色。在表达两种不同XFP的双转基因小鼠中，有可能在单个动物中差异标记3个神经元亚群。
We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in their entirety, including axons, nerve terminals, dendrites, and dendritic spines. Remarkably, each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thy1 gene). For example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglion cells or only layer 5 cortical pyramids were labeled in others. In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal.