Matrix addition by condensation for matrix-assisted laser desorption/ionization of collected aerosol particles.
Matrix addition by condensation for matrix-assisted laser desorption/ionization of collected aerosol particles.
复制标题
通过冷凝进行基质添加，用于收集的气溶胶颗粒的基质辅助激光解吸/电离。
DOI：
10.1021/ac020181i
复制
发表时间：
2002
影响因子：
7.4
通讯作者：
Murray,KermitK
中科院分区：
文献类型：
--
作者：
Jackson,ShelleyN;Murray,KermitK
紫外吸收液体基质在气溶胶颗粒上的冷凝被用来增强大分子的电离效率。用基质涂覆热解产生的颗粒，沉积在样品靶上，并通过激光解吸质谱法进行分析，没有其他基质添加。气溶胶在Collison雾化器中产生，并且颗粒在扩散干燥器中干燥，然后进入用液体基质3-硝基苄醇（NBA）饱和的加热区域，然后进入冷却的冷凝区域。在样品靶上收集基质涂覆的颗粒，并使用337 nm激光和飞行时间质谱仪进行分析。在有和没有基质添加步骤的情况下分析含有短杆菌肽S和短杆菌肽D的颗粒。基质的缩合添加增加了生物分子离子信号，并导致质谱具有较少的碎片和低质量离子干扰。
Condensation of an ultraviolet absorbing liquid matrix onto aerosol particles was used to enhance the ionization efficiency of large molecules. Laboratory-generated particles were coated with matrix, deposited on a sample target, and analyzed by laser desorption mass spectrometry with no other matrix addition. The aerosol was generated in a Collison nebulizer, and the particles were dried in a diffusion dryer before entering a heated region saturated with the liquid matrix 3-nitrobenzyl alcohol (NBA) and then entering a cooled condensation region. Matrix-coated particles were collected on a sample target and analyzed using a 337-nm laser and a time-of-flight mass spectrometer. Particles containing the peptides gramicidin S and gramicidin D were analyzed both with and without the matrix addition step. Condensation addition of matrix increased the biomolecule ion signal and resulted in mass spectra with less fragmentation and low-mass ion interference.