Integrated sophorolipid production and gravity separation
Integrated sophorolipid production and gravity separation
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DOI：
10.1016/j.procbio.2016.12.021
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发表时间：
2017-03-01
影响因子：
4.4
通讯作者：
Winterburn, James B.
中科院分区：
文献类型：
--
作者：
Dolman, Ben M.;Kaisermann, Candice;Winterburn, James B.
开发了一种从发酵液中集成重力分离槐糖脂的新方法，能够去除密度高于或低于大量发酵液的槐糖脂相，同时细胞和其他培养基成分再循环并返回生物反应器。该分离系统回收富集槐糖脂产物相的能力在使用假丝酵母的三种槐糖脂生产补料分批发酵中得到了证明，在保持槐糖脂产量的同时，所需的发酵罐体积减少了 11%。在富集度高达 9 的情况下，整个发酵过程中槐糖脂回收率高达 86%（280 克）。此外，通过去除槐糖脂相实现的肉汤粘度降低使混合功率降低了 34%，以在发酵结束时保持相同的溶解氧水平，在发酵过程中平均降低 9%。发酵时间可以延长至 1023 小时，从而可以从 1 L 初始批次体积生产 623 g 槐糖脂。这些好处可能会导致槐糖脂生产成本大幅降低，从而使提高石油采收率等大批量应用在经济上变得可行。 (C) 2017 Elsevier Ltd. 保留所有权利。
A novel method for the integrated gravity separation of sophorolipid from a fermentation broth has been developed, enabling removal of a sophorolipid phase of either higher or lower density than the bulk fermentation broth, while cells and other media components are recirculated and returned to the bioreactor. The capability of the separation system to recover an enriched sophorolipid product phase was demonstrated on three sophorolipid producing fed batch fermentations using Candida bombicola, giving an 11% reduction in fermenter volume required whilst maintaining sophorolipid production. Sophorolipid recoveries of up to 86% (280 g) of the total produced over a whole fermentation were achieved at an enrichment of up to 9. Furthermore, the broth viscosity reduction achieved by removal of the sophorolipid phase enabled a 34% reduction in mixing power to maintain the same dissolved oxygen level by the end of the fermentation, with a 9% average reduction over the course of the fermentation. Fermentation duration could be extended to 1023 h, allowing production of 623 g sophorolipid from 1 I initial batch volume. These benefits could lead to a substantial decrease in the cost of sophorolipid production, making high volume applications such as enhanced oil recovery economically feasible. (C) 2017 Elsevier Ltd. All rights reserved.