The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems.
The expression of a mountain cedar allergen comparing plant-viral apoplastic and yeast expression systems.
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山雪松过敏原的表达比较植物病毒质外体和酵母表达系统。
DOI：
10.1007/s10529-008-9665-x
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发表时间：
2008
影响因子：
2.7
通讯作者：
Kearney,ChristopherM
中科院分区：
文献类型：
--
作者：
Moehnke,MarcieH;Midoro-Horiuti,Terumi;Goldblum,RandallM;Kearney,ChristopherM
Jun A 3是雪松花粉中的一种主要过敏性蛋白，会导致过敏者患季节性过敏性鼻炎。重组Jun A 3在烟叶间质液(30 0μg/g叶料)和巴斯德毕赤酵母(10 0μg/ml)中得到了表达。过敏患者血清中的抗Jun A3多克隆抗体和IgE抗体均可与重组蛋白反应。在这两个系统中，来自植物质外体的重组蛋白含有较少的污染蛋白。这种方法可以更方便和廉价地表达重组变应原，这将允许进一步的结构研究，并可能被证明在雪松过敏的诊断和/或免疫治疗策略中有用。
Jun a 3, a major allergenic protein in mountain cedar pollen, causes seasonal allergic rhinitis in hypersensitive individuals. Recombinant Jun a 3 was expressed inNicotiana benthamianainterstitial fluid (300 μg/g leaf material) andPichia pastoris(100 μg/ml media). Polyclonal anti-Jun a 3 and IgE antibodies from the sera of allergic patients both reacted with the recombinant protein. Of the two systems, recombinant protein from the plant apoplast contained fewer contaminating proteins. This method allows for a more convenient and inexpensive expression of the recombinant allergen, which will allow for further structural studies and may prove useful in diagnostic and/or immunotherapeutic strategies for cedar allergy.