Epitope mapping of human thyroglobulin. Heterogeneous recognition by thyroid pathologic sera.
Epitope mapping of human thyroglobulin. Heterogeneous recognition by thyroid pathologic sera.
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DOI：
10.4049/jimmunol.145.11.3692
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发表时间：
1990-12
影响因子：
4.4
通讯作者：
M. Henry;Y. Malthiéry;E. Zanelli;B. Charvet
中科院分区：
文献类型：
--
作者：
M. Henry;Y. Malthiéry;E. Zanelli;B. Charvet
甲状腺球蛋白是参与自身免疫性病理的甲状腺的主要抗原。用兔抗原核表达融合蛋白的多克隆免疫血清进行抗原表位定位。筛选后的初始人甲状腺球蛋白cDNA文库和免疫反应性克隆的亚克隆，7个表位的特点和本地化的人甲状腺球蛋白单体分子。一个靠近分子的两端，另外五个集中在中间，覆盖了这条2748个氨基酸链的六分之一。18个不同的甲状腺病变的自身免疫血清的免疫反应性进行了测试，对7个先前表征的表位。桥本氏甲状腺炎的甲状腺细胞免疫反应性最强。免疫应答对于来自不同病理学的血清以及来自相同病理学的血清是异质的。然而，中心表位和近C-末端表位是最常被免疫血清识别的表位。这些发现表明，一些自身表位重叠准确地与一些异源表位，其特征在于针对成熟蛋白的多克隆免疫血清。
Thyroglobulin is the major Ag of the thyroid gland involved in autoimmune pathologies. Epitope mapping was carried out with a rabbit polyclonal immune serum against fusion proteins expressed in prokaryotic cells. After screening of an initial human thyroglobulin cDNA library and subcloning of immunoreactive clones, seven epitopes were characterized and localized on the human thyroglobulin monomeric molecule. One was close to each extremity of the molecule, and five others were concentrated in the middle, covering a sixth of this 2748-amino-acid chain. The immunoreactivities of 18 autoimmune sera from different thyroid pathologies were tested against the seven previously characterized epitopes. Those from Hashimoto's thyroiditis were the most immunoreactive. Immune responses were heterogeneous for sera from different pathologies as well as for those from the same pathology. The central epitopes and the near-C-terminal epitope, however, were the epitopes most often recognized by the immune sera. These findings show that some autoepitopes overlap accurately with some heteroepitopes characterized by a polyclonal immune serum directed against the mature protein.