Cultured autologous human cells for hard tissue regeneration: Preparation and characterization of mesenchymal stem cells from bone marrow
Cultured autologous human cells for hard tissue regeneration: Preparation and characterization of mesenchymal stem cells from bone marrow
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DOI：
10.1111/j.1525-1594.2004.07320.x
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Ohgushi, H
中科院分区：
文献类型：
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作者：
Kotobuki, N;Hirose, M;Ohgushi, H
间充质干细胞（Mesenchymal stem cells，MSCs）是一种多能细胞，在体内外均可诱导分化为中胚层细胞，也可分化为内胚层细胞或外胚层细胞。这种能力表明MSC用于组织工程的有用性。使用各种类型的CD抗体的细胞表面抗原分析表明，来自新鲜人骨髓的贴壁成纤维细胞是间充质类型，并且细胞显示出广泛的增殖和/或分化能力。我们将贴壁培养的骨髓细胞标记为MSC，并且显著地发现MSC甚至可以从老化的骨髓细胞增殖。经过大约16天的培养，我们能够从仅3 ml的新鲜人骨髓中收获1亿个MSC。MSCs在-80 ℃冻存后，其活性和成骨分化能力无明显下降。这些结果表明，骨髓间充质干细胞有望用于硬组织再生。
Mesenchymal stem cells (MSCs) are multipotent cells and can be induced in vitro and in vivo to differentiate not only into the variety of mesodermal cells, but into either ectodermal or endodermal cells. This capability indicates the usefulness of MSCs for tissue engineering. Cell surface antigen analyses using various types of CD antibodies demonstrated that adherent fibroblastic cells derived from fresh human bone marrow are mesenchymal types and the cells showed extensive capability for proliferation and/or differentiation. We labeled the adherent cultured marrow cells as MSCs and, significantly, found the MSCs could even proliferate from aged marrow cells. After about sixteen days of culturing, we were able to harvest 100 million MSCs from only 3 ml of fresh human marrow. Moreover, the MSCs could be cryopreserved at -80degreesC without noticeable loss of viability and capability of osteoblastic differentiation. These results indicate that MSCs hold promise for utilization in hard tissue regeneration.