Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339
Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339
复制标题
DOI：
10.1194/jlr.d500019-jlr200
复制
发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Billheimer, JT
中科院分区：
文献类型：
--
作者：
Millar, JS;Cromley, DA;Billheimer, JT
甘油三酯（TG）是一种不溶于水的富含能量的脂质，由肝脏分泌，作为极低密度脂蛋白（VLDL）的一部分，为肝外组织提供能量。VLDL的过度产生与心血管心脏病的风险增加有关;这重新引起了人们对影响肝脏TG产生的因素的兴趣。通过测量在脂蛋白脂肪酶（LPL）的TG水解被抑制的条件下血浆TG的时间增加来确定TG产生速率。为此目的，非离子去污剂Triton WR-1339（Triton）通常用于抑制LPL。Triton除了抑制TG水解外，还具有可能对脂蛋白代谢产生不利影响的特性。另一种非离子去污剂泊洛沙姆407（P-407）也能抑制LPL。在这些研究中，我们证明P-407在测定TG产生方面与Triton相当，但没有Triton的不良副作用。
Triglyceride (TG), a water-insoluble energy-rich lipid, is secreted by the liver as part of very low density lipoproteins (VLDLs) to supply energy to extrahepatic tissues. Overproduction of VLDL is associated with increased risk of cardiovascular heart disease; this has renewed an interest in factors that affect hepatic TG production. The TG production rate is determined by measuring temporal increases in plasma TG under conditions in which TG hydrolysis by lipoprotein lipase (LPL) is inhibited. The nonionic detergent, Triton WR-1339 (Triton), has commonly been used to inhibit LPL for this purpose. Triton, in addition to inhibition of TG hydrolysis, has properties that have the potential to adversely influence lipoprotein metabolism. Another nonionic detergent, poloxamer 407 (P-407), also inhibits LPL. In these studies, we demonstrate that P-407 is comparable to Triton in the determination of TG production but without the unwanted side effects of Triton.