Brain energy metabolism in streptozotocin-diabetes.
Brain energy metabolism in streptozotocin-diabetes.
复制标题
链脲佐菌素糖尿病的脑能量代谢。
DOI：
10.1042/bj2490057
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hawkins,RA
中科院分区：
文献类型：
--
作者：
Mans,AM;DeJoseph,MR;Davis,DW;Hawkins,RA
通过使用[6- 14 C]葡萄糖和定量放射自显影术，测量了链脲佐菌素诱导的糖尿病大鼠（65 mg/kg静脉注射）1周或4周持续时间的局部脑葡萄糖使用。测量血浆和脑中几种代谢物的浓度。结果与正常未处理大鼠的结果进行比较。血浆和脑中的葡萄糖浓度均升高，两个糖尿病组的升高程度相似。对照组、1周组和4周组的血浆酮体浓度分别为0.25、1.0和3.15 μ mol/ml（乙酰乙酸和3-羟基丁酸之和）。糖尿病1周后，整个大脑的葡萄糖使用量增加（59个大脑区域中的55个差异具有统计学意义），整个大脑增加了25%。相比之下，糖尿病4周后整个大脑的正常率。在两个糖尿病组中，没有一个大脑区域受到明显不同的影响。在实验期间，14 C作为乳酸盐或丙酮酸盐没有显著损失，也没有任何组中乳酸盐净产生的迹象。同样排除了可能影响糖尿病大鼠结果的其他方法学考虑。由于酮体被认为是补充葡萄糖作为大脑的代谢燃料，我们的研究结果表明，在链脲佐菌素糖尿病期间，大脑能量消耗增加。
Regional brain glucose use was measured in rats with streptozotocin-induced diabetes (65 mg/kg intravenously) of 1 or 4 weeks duration, by using [6-14C]glucose and quantitative autoradiography. The concentrations of several metabolites were measured in plasma and brain. Results were compared with those from normal untreated rats. Glucose concentrations were increased in both plasma and brain, to similar degrees in both diabetic groups. Plasma ketone-body concentrations were 0.25, 1.0, and 3.15 mumol/ml in the control, 1-week and 4-week groups respectively (sum of acetoacetate and 3-hydroxybutyrate). Glucose use was increased throughout the brain (differences were statistically significant in 55 of 59 brain areas) after 1 week of diabetes, with an increase of 25% for the brain as a whole. In contrast, normal rates were found throughout the brain after 4 weeks of diabetes. None of the brain areas was affected significantly differently from the others, in either diabetic group. There was no significant loss of 14C as lactate or pyruvate during the experimental period, nor was there any indication of net production of lactate in any of the groups. Other methodological considerations that could have affected the results obtained in the diabetic rats were likewise ruled out. Because the ketone bodies are expected to supplement glucose as a metabolic fuel for the brain, our results indicate that brain energy consumption is increased during streptozotocin-diabetes.