Molecular cloning of Elk-3, a new member of the Ets family expressed during mouse embryogenesis and analysis of its transcriptional repression activity
Molecular cloning of Elk-3, a new member of the Ets family expressed during mouse embryogenesis and analysis of its transcriptional repression activity
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DOI：
10.1089/dna.1996.15.855
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发表时间：
1996-10-01
影响因子：
3.1
通讯作者：
Fujimura, Y
中科院分区：
文献类型：
--
作者：
Nozaki, M;Onishi, Y;Fujimura, Y
我们从16天的小鼠胚胎中克隆了一个编码新的Ets转录因子的cDNA克隆Elk-3，其推导的蛋白质的氨基酸序列与人ELK-1和SAP-1同源。该蛋白与ELK-1和SAP-1具有一些共同的结构特征，如氨基端的Ets DNA结合位点、血清反应因子相互作用结构域和羧基端丝氨酸或苏氨酸残基的磷酸化位点。北方印迹显示，4和2.1 kb的两个转录本在成年卵巢和肺中表达，2.1 kb的转录本在8- 14天的胚胎中占主导地位。我们检测了Elk-3蛋白在含有Ets结合位点的细胞角蛋白EndoA增强子上的转录活性。Elk-3蛋白强烈抑制增强子活性，但在没有增强子的情况下不影响基础启动子的活性。此外，Elk-3可以抑制Ets-2作为EndoA增强子上的转录激活因子的活性。这些数据表明，Elk-3基因产物在胚胎发生过程中的转录调控中发挥作用。
We isolated a cDNA clone, Elk-3, that encodes a novel Ets transcription factor from 16-day mouse embryos, The deduced amino acid sequence of the protein was homologous to human ELK-1 and SAP-1. This protein, ELK-1, and SAP-1 shared some unique structural properties such as an Ets DNA-binding site in the aminoterminal region, a serum response factor interacting domain and phosphorylation sites of serine or threonine residues in the carboxy-terminal region. Northern blotting weakly revealed that two transcripts of 4 and 2.1 kb are expressed in the adult ovary and lung and a 2.1-kb transcript predominated in 8- to 14-day embryos. We assayed the transcriptional activities of Elk-3 protein on the cytokeratin EndoA enhancer containing Ets binding sites in endodermal cells. Elk-3 protein strongly repressed enhancer activity but did not affect the activity of the basal promoter in the absence of the enhancer. Furthermore, Elk-3 can suppress the activity of Ets-2 as the transcriptional activator on the EndoA enhancer. These data suggested that the Elk-3 gene product plays a role in transcriptional regulation during embryogenesis.