ISOLATION OF ANAEROBIC BACTERIA FROM HUMAN GINGIVA AND MOUSE CECUM BY MEANS OF A SIMPLIFIED GLOVE BOX PROCEDURE
ISOLATION OF ANAEROBIC BACTERIA FROM HUMAN GINGIVA AND MOUSE CECUM BY MEANS OF A SIMPLIFIED GLOVE BOX PROCEDURE
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DOI：
10.1128/aem.17.4.568-576.1969
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
FRETER, R
中科院分区：
文献类型：
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作者：
ARANKI, A;SYED, SA;FRETER, R
描述了一种由透明柔性乙烯基塑料制成的厌氧手套箱。它价格低廉，操作简单，不仅可用于研究，而且无需经过专门培训的技术人员也可用于临床实验室。常规细菌学技术可在手套箱内用于培养和转移厌氧细菌。盒子可以加热到37℃，因此也可以作为厌氧培养箱，允许在任何时候检查培养物。培养基可以准备和琼脂板可以倒在实验室工作台上以传统的方式。将微量的钯黑色催化剂覆盖在电镀的琼脂培养基上，在引入手套箱后的2天内将培养基降低到- 300 mv的氧化还原（O-R）电位。尽管该系统更简单，但在所有测试参数方面，该系统与滚管法相当或优于滚管法，包括培养基中可获得的O-R电位、气相o2浓度以及从小鼠盲肠中分离厌氧细菌的效率。对比研究表明，传统的厌氧罐法不适合从人牙龈标本和小鼠盲肠中分离严格厌氧菌。这是由于试样和介质暴露在空气中，在开放的实验室工作台上电镀。厌氧罐足以维持厌氧细菌生长的适当条件，一旦这些在手套箱中建立。图片
An anaerobic glove box constructed of clear flexible vinyl plastic is described. It is sufficiently inexpensive and simple in operation to be used not only in research but also in a clinical laboratory by technicians without special training. Conventional bacteriological techniques may be used inside the glove box for culturing and transferring anaerobic bacteria. The box may be heated to 37 C and thus serve as an anaerobic incubator as well, permitting inspection of cultures at any time. Media may be prepared and agar plates may be poured on the laboratory bench in the conventional manner. An overlay of trace amounts of palladium black catalyst over plated agar media reduces the medium to an oxidation-reduction (O-R) potential of - 300 mv within 2 days after introduction into the glove box. In spite of its greater simplicity, the system matched or excelled the roll tube method with respect to all parameters tested, including O-R potential obtainable in the media, O2concentration in the gas phase, and efficiency in isolating anaerobic bacteria from the mouse cecum. Comparative studies indicate that the conventional anaerobic jar method was inadequate for the isolation of strict anaerobes from human gingival specimens and from the mouse cecum. This was due to the exposure of specimens and media to air during plating on the open laboratory bench. Anaerobic jars were adequate for maintaining the proper conditions for growth of anaerobic bacteria once these had been established in the glove box.Images