Genomic and proteomic analysis of phEco32, a novel Escherichia coli bacteriophage
Genomic and proteomic analysis of phEco32, a novel Escherichia coli bacteriophage
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DOI：
10.1016/j.jmb.2007.12.077
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发表时间：
2008-03-28
影响因子：
5.6
通讯作者：
Severinov, Konstantin
中科院分区：
文献类型：
--
作者：
Savalia, Dhruti;Westblade, Lars F.;Severinov, Konstantin
在大规模筛选可用于治疗牛乳腺炎的噬菌体时分离出一种感染大肠杆菌的新型噬菌体。该噬菌体的 77,554 bp 基因组被命名为 phiEco32，已被测序和注释，并通过电子显微镜和蛋白质组学对其病毒粒子进行了表征。鉴定出两种与宿主 RNA 聚合酶相互作用的 phiEco32 编码蛋白。其中之一是 ECF 家族 sigma 因子，它可能负责某些病毒基因的转录。另一种RNA聚合酶结合蛋白是一种新型转录抑制剂，其作用机制仍有待确定。 (C) 2008 Elsevier Ltd. 保留所有权利。
A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined. (C) 2008 Elsevier Ltd. All rights reserved.