Regulation of Unperturbed DNA Replication by Ubiquitylation.
Regulation of Unperturbed DNA Replication by Ubiquitylation.
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DOI：
10.3390/genes6030451
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发表时间：
2015-06-25
期刊：
影响因子：
3.5
通讯作者：
Gambus A
中科院分区：
文献类型：
--
作者：
Moreno SP;Gambus A
蛋白质的翻译后修饰通过连接小球状蛋白泛素（即，泛素化）代表了真核细胞所采用的最丰富和最通用的蛋白质调节机制之一。泛素化影响几乎每一个细胞过程，其在协调DNA损伤反应中的关键作用已得到充分证实。在这篇综述中，我们重点关注泛素化控制未受干扰的DNA复制过程的方式。我们总结了积累的知识，显示了泛素驱动的蛋白质降解的主导作用，在建立有利于复制起点许可和S期进入的条件。重要的是，我们还提出了新出现的主要作用的泛素化的协调活动的DNA复制过程：防止再复制，调节DNA复制叉的进展，染色质重建和拆卸的复制体在终止复制叉。
Posttranslational modification of proteins by means of attachment of a small globular protein ubiquitin (i.e., ubiquitylation) represents one of the most abundant and versatile mechanisms of protein regulation employed by eukaryotic cells. Ubiquitylation influences almost every cellular process and its key role in coordination of the DNA damage response is well established. In this review we focus, however, on the ways ubiquitylation controls the process of unperturbed DNA replication. We summarise the accumulated knowledge showing the leading role of ubiquitin driven protein degradation in setting up conditions favourable for replication origin licensing and S-phase entry. Importantly, we also present the emerging major role of ubiquitylation in coordination of the active DNA replication process: preventing re-replication, regulating the progression of DNA replication forks, chromatin re-establishment and disassembly of the replisome at the termination of replication forks.