Effects of FK506 on nerve regeneration and reinnervation after graft or tube repair of long nerve gaps
Effects of FK506 on nerve regeneration and reinnervation after graft or tube repair of long nerve gaps
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DOI：
10.1002/mus.1088
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发表时间：
2001-07-01
期刊：
影响因子：
3.4
通讯作者：
Gold, BG
中科院分区：
文献类型：
--
作者：
Navarro, X;Udina, E;Gold, BG
我们比较了 FK506 给药对坐骨神经切除和使用自体移植物或使用在小鼠体内留下 6 毫米间隙的硅胶管进行修复后的再生和神经支配的影响。通过无创方法评估神经支配功能，以确定术后 4 个月内后爪运动、感觉和出汗功能的恢复情况。在随访结束时对再生神经进行形态测量分析。与硅胶管修复相比，神经移植可以在测试的四种功能中实现更快、更高水平的神经再支配。 FK506 治疗（仅前 9 周）导致神经支配的开始和自体移植后实现的最大程度的恢复略有改善，但并不显着。直接依赖于轴突再生的数字神经痛觉和复合神经动作电位的恢复，与肌肉和汗腺的神经支配相比，FK506 的进展更好，肌肉和汗腺的神经支配需要重建与靶细胞的突触接触。在 FK506 治疗的大鼠中，再生神经中的有髓纤维表现出更成熟的外观。然而，FK506 在再生受限的情况下表现出边际效应，例如在小鼠坐骨神经中桥接 6 毫米间隙的硅胶管中。总之，FK506 治疗提高了神经切除和自体移植修复后的功能恢复率。 (C) 2001 约翰威利父子公司
We compared the effects of FK506 administration on regeneration and reinnervation after sciatic nerve resection and repair with an autologous graft or with a silicone tube leaving a 6-mm gap in the mouse. Functional reinnervation was assessed by noninvasive methods to determine recovery of motor, sensory, and sweating functions in the hindpaw over 4 months after operation. Morphometric analysis of the regenerated nerves was performed at the end of follow-up. The nerve graft allowed for faster and higher levels of reinnervation in the four functions tested than silicone tube repair. Treatment with FK506 (for the first 9 weeks only) resulted in a slight, although not significant, improvement of the onset of reinnervation and of the maximal degree of recovery achieved after autografting. The recovery of pain sensibility and of the compound nerve action potentials in the digital nerves, which directly depend on axonal regeneration, showed better progression with FK506 than reinnervation of muscles and sweat glands, which require reestablishment of synaptic contacts with target cells. The myelinated fibers in the regenerated nerve showed a more mature appearance in the FK506-treated rats. However, FK506 showed a marginal effect in situations in which regeneration was limited, as in a silicone tube bridging a 6-mm gap in the mouse sciatic nerve. In conclusion, treatment with FK506 improved the rate of functional recovery after nerve resection and autograft repair. (C) 2001 John Wiley & Sons, Inc.