THE INTERACTION OF PLASMA-PROTEINS WITH POLYMERS .1. RELATIONSHIP BETWEEN POLYMER SURFACE-ENERGY AND PROTEIN ADSORPTION-DESORPTION
THE INTERACTION OF PLASMA-PROTEINS WITH POLYMERS .1. RELATIONSHIP BETWEEN POLYMER SURFACE-ENERGY AND PROTEIN ADSORPTION-DESORPTION
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DOI：
10.1002/jbm.820140406
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
LYMAN, DJ
中科院分区：
文献类型：
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作者：
BASZKIN, A;LYMAN, DJ
牛白蛋白的吸附，本文用~（125）I标记的蛋白质研究了几种聚合物膜上的纤维蛋白原和球蛋白的吸附。测定了蛋白质化聚合物膜在牛血浆中的解吸动力学。这些相同的聚合物上的接触角测量允许的聚合物-蛋白质溶液系统的分散（WAd）和极性（IP）的组件的计算。从这些测量的结果表明，在聚合物-蛋白质溶液界面的非分散-分散力平衡，表示的IP/WAd的比率，是一个重要的因素结合的蛋白质聚合物表面。纤维蛋白原的纯度和聚合物表面的清洁程序影响吸附在聚合物表面上的蛋白质的绝对值。
Adsorption of bovine albumin, .gamma.-globulin and fibrinogen from phosphate buffer solution (pH = 7.5) onto several polymer films was studied using the radioiodinated proteins (125I). The kinetics of desorption of the proteinated polymer films in bovine plasma was determined. Contact angle measurements on these same polymers allowed the calculation of dispersive (WAd) and polar (Ip) components of the polymer-protein solution system. Results from these measurements show that the nondispersive-dispersive force balance at the polymer-protein solution interface, expressed by the Ip/WAd ration, is an important factor for binding of proteins on polymer surfaces. The purity of fibrinogen and the cleaning procedures for the polymer surfaces influence the absolute values of proteins adsorbed on polymer surfaces.