Complete Sequence and Gene Organization of the Mitochondrial Genome of Tokay (Gekko gecko)
Complete Sequence and Gene Organization of the Mitochondrial Genome of Tokay (Gekko gecko)
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发表时间：
2005-12
影响因子：
4.9
通讯作者：
Demin Han;K. Zhou
中科院分区：
文献类型：
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作者：
Demin Han;K. Zhou
采用长PCR扩增、克隆和引物行走测序方法确定了大壁虎线粒体基因组的完整序列。基因组大小为16 435 bp，包含13个蛋白质编码基因、2个核糖体基因和22个转移RNA基因。 Gekko 的 mt 基因组在基因组成、顺序、方向、tRNA 结构、鸟嘌呤百分比低、胸腺嘧啶百分比高以及碱基 GC 和 AT 的偏差等方面与大多数脊椎动物相似。碱基 A 在第三个密码子位置上是首选，因为蛋白质基因与两栖动物和鱼类而不是羊膜脊椎动物相似。标准终止码（TAA）仅存在于三个蛋白质基因中，比大多数脊椎动物的少。转移RNA基因的长度范围为63至76 nt，其平面结构呈现典型的三叶草叶状，除了tRNA-Cys和tRNA-Ser (AGY)由于缺乏D臂之外。
Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mt genome of Gekko is similar to most of the vertebrates in gene components, order, orientation, tRNA structures, low percentage of guanine and high percentage of thymine, and skews of base GC and AT. Base A was preferred at third codon positions for protein genes is similar to amphibians and fishes rather than amnion vertebrates. The standard stop codes (TAA) present only in three protein genes, less than those of most vertebrates. Transfer RNA genes range in length from 63 to 76 nt, their planar structure present characteristic clover leaf, except for tRNA-Cys and tRNA-Ser (AGY) because of lacking the D arm.