Altered distribution of β-catenin and prognostic roles in colorectal carcinogenesis
Altered distribution of β-catenin and prognostic roles in colorectal carcinogenesis
复制标题
DOI：
10.1080/00365520701785194
复制
发表时间：
2008-01-01
影响因子：
1.9
通讯作者：
Sun, Yujie
中科院分区：
文献类型：
--
作者：
Chen, Senqing;Liu, Juying;Sun, Yujie
目标。尽管β -连环蛋白细胞质稳定和核易位在结直肠癌（CRC）的发生中起着关键作用，但其机制尚不清楚。本研究旨在探讨环氧化酶(COX)-2和E-cadherin的表达以及β -catenin基因外显子3突变与β -catenin分布改变的关系及其在结直肠癌进展和预后中的作用。材料和方法。采用免疫组化方法检测96份组织标本中β -catenin、COX-2和E-cadherin的表达，采用聚合酶链式反应（PCR）和变性高效液相色谱法（DHPLC）筛选β -catenin基因外显子3突变位点。结果。细胞质/细胞核中β -catenin的表达和膜上E-cadherin表达的减少分别与序贯结直肠癌发生的早期和晚期相关(p
Objective. Although beta-catenin cytoplasmic stabilization and nuclear translocation play a key role in initiation of colorectal cancer (CRC), the mechanisms are far from clear. The aim of this study was to investigate the relation of expressions of cyclooxygenase (COX)-2 and E-cadherin, and the beta-catenin gene exon 3 mutation to the altered distribution of beta-catenin, and their roles in CRC progression and prognosis. Material and methods. Expressions of beta-catenin, COX-2 and E-cadherin in 96 tissue specimens were detected by immunohistochemistry, and mutation of beta-catenin gene exon 3 was screened by polymerase chain reaction (PCR) and denaturing high-performance liquid chromatography (DHPLC). Results. Cytoplasmic/nuclear expression of beta-catenin and reduced membranous expression of E-cadherin were associated, respectively, with the earlier and later stages of sequential colorectal carcinogenesis (p