p-Nonyl-phenol: an estrogenic xenobiotic released from "modified" polystyrene.
p-Nonyl-phenol: an estrogenic xenobiotic released from "modified" polystyrene.
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DOI：
10.1289/ehp.9192167
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发表时间：
1991-05
影响因子：
10.4
通讯作者：
Sonnenschein C
中科院分区：
文献类型：
--
作者：
Soto AM;Justicia H;Wray JW;Sonnenschein C
烷基酚广泛用作塑料添加剂和表面活性剂。我们报告的烷基酚，壬基酚，作为从塑料离心管释放的雌激素物质的鉴定。该化合物用甲醇提取，经快速色谱和反相高效液相色谱纯化，并通过气相色谱-质谱法鉴定。壬基酚诱导人雌激素敏感的MCF 7乳腺肿瘤细胞增殖和孕激素受体。壬基酚还引发大鼠子宫内膜中的有丝分裂活性;该结果证实了MCF 7细胞增殖生物测定的可靠性。烷基酚，特别是壬基酚的雌激素特性表明，在实验和诊断测试中使用含有这些化学品的塑料器皿可能会导致虚假结果，这些化合物以及烷基酚聚乙氧基化物也可能对暴露的人类和环境造成潜在危害。
Alkylphenols are widely used as plastic additives and surfactants. We report the identification of an alkylphenol, nonylphenol, as an estrogenic substance released from plastic centrifuge tubes. This compound was extracted with methanol, purified by flash chromatography and reverse-phase high performance liquid chromatography, and identified by gas chromatography-mass spectrometry. Nonylphenol induced both cell proliferation and progesterone receptor in human estrogen-sensitive MCF7 breast tumor cells. Nonylphenol also triggered mitotic activity in rat endometrium; this result confirms the reliability of the MCF7 cell proliferation bioassay. The estrogenic properties of alkylphenols, specifically nonylphenols, indicate that the use of plasticware containing these chemicals in experimental and diagnostic tests may lead to spurious results, and these compounds as well as alkylphenol polyethoxylates may also be potentially harmful to exposed humans and the environment at large.