Uncoupling salicylic acid-dependent cell death and defense-related responses from disease resistance in the Arabidopsis mutant acd5.
Uncoupling salicylic acid-dependent cell death and defense-related responses from disease resistance in the Arabidopsis mutant acd5.
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DOI：
10.1093/genetics/156.1.341
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发表时间：
2000-09
期刊：
影响因子：
3.3
通讯作者：
J. Greenberg;F. P. Silverman;Hua Liang
中科院分区：
文献类型：
--
作者：
J. Greenberg;F. P. Silverman;Hua Liang
水杨酸（SA）是高等植物对多种病害的抗性所必需的。SA依赖性细胞死亡和防御相关反应与抗病性相关。拟南芥的加速细胞死亡5突变体提供了额外的遗传证据，SA调节细胞死亡和防御相关的反应。然而，在acd 5中，这些事件与抗病性无关。acd 5植物在发育早期更容易受到假单胞菌的影响，并在发育后期显示自发的SA积累、细胞死亡和防御相关标记。在acd5植物中，细胞死亡和防御相关的反应是SA依赖性的，但它们不赋予抗病性。双突变体acd 5和nonexpressor的PR1，其中SA信号被部分阻断，显示大大减弱细胞死亡，表明NPR1在控制细胞死亡的作用。激素乙烯增强SA的作用，并且对于拟南芥中的疾病症状发展是重要的。acd 5和乙烯不敏感2的双突变体，其中乙烯信号被阻断，显示细胞死亡减少，支持乙烯在细胞死亡控制中的作用。我们建议，acd 5植物模仿P.感染的野生型植物和SA和乙烯通常参与调节细胞死亡在一些敏感的病原体感染。
Salicylic acid (SA) is required for resistance to many diseases in higher plants. SA-dependent cell death and defense-related responses have been correlated with disease resistance. The accelerated cell death 5 mutant of Arabidopsis provides additional genetic evidence that SA regulates cell death and defense-related responses. However, in acd5, these events are uncoupled from disease resistance. acd5 plants are more susceptible to Pseudomonas syringae early in development and show spontaneous SA accumulation, cell death, and defense-related markers later in development. In acd5 plants, cell death and defense-related responses are SA dependent but they do not confer disease resistance. Double mutants with acd5 and nonexpressor of PR1, in which SA signaling is partially blocked, show greatly attenuated cell death, indicating a role for NPR1 in controlling cell death. The hormone ethylene potentiates the effects of SA and is important for disease symptom development in Arabidopsis. Double mutants of acd5 and ethylene insensitive 2, in which ethylene signaling is blocked, show decreased cell death, supporting a role for ethylene in cell death control. We propose that acd5 plants mimic P. syringae-infected wild-type plants and that both SA and ethylene are normally involved in regulating cell death during some susceptible pathogen infections.