Molecular cloning and characterization of four cDNAs encoding the isoforms of NAD-dependent sorbitol dehydrogenase from the Fuji apple
Molecular cloning and characterization of four cDNAs encoding the isoforms of NAD-dependent sorbitol dehydrogenase from the Fuji apple
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DOI：
10.1016/s0168-9452(01)00599-4
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发表时间：
2002-04-01
期刊：
影响因子：
5.2
通讯作者：
Chung, WI
中科院分区：
文献类型：
--
作者：
Park, SW;Song, KJ;Chung, WI
我们从苹果属植物中分离到四个编码不同NAD-SDH亚型的cDNA。富士。MdSDH1、MdSDH2、MdSDH3和MdSDH4的氨基酸序列与人、大鼠和绵羊的NAD-SDH有较高的同源性，MdSDH1与已报道的苹果NAD-SDH几乎相同。MdSDH2、MdSDH3和MdSDH4与MdSDH1的一级结构有明显的区别，与MdSDH1的同源性为69%-71%，与MdSDH2的表达同源性为90%-92%。MdSDH3和MdSDH4仅限于下沉组织，包括幼叶、茎、根和成熟的果实。暗示它们与苹果组织的库活性有关。与其他不同的是，MdSDH1在源叶和幼叶中高表达，表明MdSDH1的表达并不局限于库区组织。在果实发育过程中，4个SDH基因在果实发育后期高表达，表明它们在果实成熟期发挥重要作用，而不是在果实发育早期。在大肠杆菌中表达的MdSDH2和MdSDH3融合蛋白具有双向的NAD-SDH活性，但不具有NADP-SDH活性。本研究表明，MdSDH1、MdSDH2、MdSDH3和MdSDH4编码依赖NAD的山梨醇脱氢酶的新亚型，属于两个独立的系统发育类群。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
We isolated four cDNAs encoding different isoforms of NAD-SDH from Malus x domestica cv. Fuji. The derived amino acid sequences of MdSDH1, MdSDH2, MdSDH3, and MdSDH4 have considerable homology with NAD-SDHs from human, rat, and sheep.,MdSDH1 is almost identical with the previously reported apple NAD-SDH. MdSDH2, MdSDH3, and MdSDH4 are clearly distinguished from MdSDH1 based on the primary structure, showing 69-71% identity with MdSDH1 and 90-92% identity with each other, The expression of MdSDH2. MdSDH3, and MdSDH4 is restricted to sink tissues including young leaves, stems, roots, and maturing fruits. implying their relationship with the sink activity of apple tissues. Unlike the others, MdSDH1 is highly expressed in source leaves as well as young leaves, indicating that the expression of MdSDH1 is not restricted to sink tissues. During the fruit development, four SDH genes are highly expressed at the late stage, suggesting that they play an important role in the period of fruit maturation rather than in the early stage of fruit development. MdSDH2 and MdSDH3 fusion proteins expressed in Escherichia coli cells exhibit NAD-SDH activity in both direction, but not NADP-SDH activity. The present study indicates that MdSDH1, MdSDH2, MdSDH3, and MdSDH4 encode the novel isoforms of NAD-dependent sorbitol dehydrogenase categorized to two independent phylogenetic groups. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.