Microevolution of dengue viruses circulating among primary school children in Kamphaeng Phet, Thailand
Microevolution of dengue viruses circulating among primary school children in Kamphaeng Phet, Thailand
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DOI：
10.1128/jvi.02728-07
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发表时间：
2008-06-01
影响因子：
5.4
通讯作者：
Endy, Timothy P.
中科院分区：
文献类型：
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作者：
Jarman, Richard G.;Holmes, Edward C.;Endy, Timothy P.
为了确定登革热病毒（DENV）在有限空间和时间尺度上的遗传变异程度和结构，我们对2001年从泰国坎phaeng Phet一项前瞻性学校研究中收集的DENY-1、-2和-3分离株的E（包膜）基因进行了测序，这些分离株来自被诊断为登革热的儿童。我们的分析揭示了病毒在时间和空间上的大量遗传变异，多个病毒谱系在单个学校中循环，表明DENV频繁的基因流进入这个微环境。对DENV-2样本的更详细分析显示，单个学校内的病毒分离株具有很强的聚类性，并且有证据表明，在空间上密切相关的学校之间，病毒基因流动更为频繁。相反，我们观察到在单个学校的多个时间点采样的病毒分离株几乎没有进化变化，表明突变固定率低。这些结果表明，频繁的病毒迁移到坎phaeng Phet，加上种群（学校）细分，在当地范围内塑造了DENV的遗传多样性，而不是在学校集水区内的原位进化。
To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENY-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with dengue disease. Our analysis revealed substantial viral genetic variation in both time and space, with multiple viral lineages circulating within individual schools, suggesting the frequent gene flow of DENV into this microenvironment. More-detailed analyses of DENV-2 samples revealed strong clustering of viral isolates within individual schools and evidence of more-frequent viral gene flow among schools closely related in space. Conversely, we observed little evolutionary change in those viral isolates sampled over multiple time points within individual schools, indicating a low rate of mutation fixation. These results suggest that frequent viral migration into Kamphaeng Phet, coupled with population (school) subdivision, shapes the genetic diversity of DENV on a local scale, more so than in situ evolution within school catchment areas.