Upregulation of caveolin-1 and SR-B1 in mice with non-alcoholic fatty liver disease
Upregulation of caveolin-1 and SR-B1 in mice with non-alcoholic fatty liver disease
复制标题
患有非酒精性脂肪肝疾病的小鼠中 Caveolin-1 和 SR-B1 的上调。
DOI：
10.1016/s1499-3872(13)60099-5
复制
发表时间：
2013-12-01
影响因子：
3.3
通讯作者：
Xu, Guo-Qiang
中科院分区：
文献类型：
--
作者：
Qiu, Yan;Liu, Shan;Xu, Guo-Qiang
背景：非酒精性脂肪性肝病（NAFLD）是肝脏疾病最常见的原因之一，患病率明显增加。然而，其机制尚不清楚。本研究旨在阐明小窝蛋白-1（caveolin-1，cav 1）和清道夫受体B类1（scavenger receptor class B type 1，SR-B1）在NAFLD中的作用。处死小鼠以收集血浆并收获肝脏;测量其血浆脂质浓度。分别采用实时定量聚合酶链反应（qPCR）和蛋白质印迹法测定肝cav 1和SR-B1 mRNA和蛋白表达。结果：高脂饲料可引起大鼠血脂升高，诱发NAFLD，肝/体重比增加，肝细胞中cav 1和SR-B1的表达增加，肝细胞中cav 1和SR-B1的表达增加，肝细胞中cav 1和SR-B1的表达增加。与对照组（n=6）相比，NAFLD小鼠（n=12）肝组织中cav 1和SR-B1的mRNA和蛋白水平均显著升高（cav 1 mRNA：1.536 ± 0.226 vs 0.980 ± 0.272，P
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is one of the most frequent causes of liver diseases, with markedly increased prevalence. However, its mechanisms are not clear. The present study was undertaken to illustrate the role of caveolin-1 (cav1) and the scavenger receptor class B type 1 (SR-B1) in NAFLD.METHODS: Adult male C57BL/6 mice were fed with a normal diet or high fat and cholesterol (HFC) diet for 14 weeks. The mice were sacrificed to collect plasma and harvest the liver; their plasma lipid concentration was measured. Hepatic cav1 and SR-B1 mRNA and protein expression were determined by real-time quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. In order to study cav1 and SR-B1 distribution and change in hepatocytes, immunohistochemical analysis was performed.RESULTS: HFC diet increased plasma lipids, induced NAFLD and increased the liver/body weight ratio. Compared to the control mice (n=6), the mRNA and protein levels of cavl and SR-B1 in liver tissue of the NAFLD mice (n=12) increased significantly (cavl mRNA: 1.536 +/- 0.226 vs 0.980 +/- 0.272, P