Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections
Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections
复制标题
DOI：
10.3389/fmicb.2019.02537
复制
发表时间：
2019-11-12
影响因子：
5.2
通讯作者：
Ramig, Robert F.
中科院分区：
文献类型：
--
作者：
Gibson, Shelley B.;Green, Sabrina I.;Ramig, Robert F.
噬菌体治疗需要良好表征的噬菌体文库。在这里，我们描述了三个目标物种：大肠杆菌，铜绿假单胞菌和阴沟肠杆菌噬菌体文库的生成。本文介绍了分离宿主的基本噬菌体特征、序列分析、生长特性、宿主范围和对一些当代临床分离株的毒力。需要这些信息，然后才能将ESTA添加到噬菌体库中，用于潜在的人类使用或在实验室之间共享，用于eIND（紧急研究性新药）下的人类同情使用方案。临床情况下，这些潜在的可用于讨论。目前正在动物模型中对本文所述的药物进行表征，并可用于eIND。
Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and virulence on a number of contemporary clinical isolates are presented. This information is required before phages can be added to a phage library for potential human use or sharing between laboratories for use in compassionate use protocols in humans under eIND (emergency investigational new drug). Clinical scenarios in which these phages can potentially be used are discussed. The phages presented here are currently being characterized in animal models and are available for eINDs.