The route of antigen delivery determines the airway and lung tissue mechanical responses in allergic rats
The route of antigen delivery determines the airway and lung tissue mechanical responses in allergic rats
复制标题
DOI：
10.1046/j.1365-2222.1999.00471.x
复制
发表时间：
1999-04-01
影响因子：
6.1
通讯作者：
Sly, PD
中科院分区：
文献类型：
--
作者：
Hall, GL;Peták, F;Sly, PD
背景以前的研究结果表明，过敏性动物吸入抗原后组织收缩。进一步的研究已经证明了吸入（i.h.）或静脉注射（i. v.）递送醋甲胆碱（MCh）。目的本研究的目的是比较i.h.后气道和实质力学的急性过敏反应。方法用卵白蛋白（OVA）致敏Brown Norway大鼠，用免疫组化方法检测其免疫原性。将大鼠麻醉、麻痹和开胸，并在对照、盐水后和任一i. h.（n = 7）或静脉内（n = 5）施用OVA。根据气道阻力（Raw）和惯性（Iaw）以及恒定相组织实质阻尼（G）和弹性（H）评估ZL。在Raw [192 +/- 32（SE）%]、G（223 +/- 21%）和H（141 +/- 5%）中发现OVA攻毒升高。Raw在静脉内激发后显示出更高的升高（418 +/- 57%），而G（278 +/- 30%）和H（130 +/- 4%）的升高大约等于吸入抗原后观察到的升高。吸入抗原可产生明显更高的气道阻力反应，而吸入抗原则导致气道和实质混合反应。
Background Previous results have shown tissue constriction in allergic animals following inhalation of an antigen. Further studies have demonstrated a differing response pattern in airway and parenchymal mechanics following inhaled (i.h.) or intravenous (i.v.) delivery of methacholine (MCh).Object ive The purpose of this study was to compare the acute allergic response in airway and parenchymal mechanics following i.h. and i.v. antigen challenge.Methods Brown Norway rats were sensitized to ovalbumin (OVA). Rats were anaesthetized, paralysed, and thoracotomized,and lung input impedance (ZL) between 0.5 and 21 Hz was measured using small-amplitude pseudo-random oscillations at control, after saline, and for up to 1 h after either i.h. (n = 7) or i.v. (n = 5) administration of OVA. ZL was evaluated in terms of airway resistance (Raw) and inertance (Iaw), and a constant phase tissue parenchymal damping (G) and elastance (H).Results Following i.h. OVA challenge elevations were found in Raw [192 +/- 32(SE) %], G (223 +/- 21%), and H (141 +/- 5%). Raw showed higher elevation after i.v. challenge (418 +/- 57%), whereas the elevation in G (278 +/- 30%) and H (130 +/- 4%) was approximately equal to those seen following inhalation of an antigen.Conclusions Delivery (i.v.) of an antigen produces a significantly higher response in airway resistance, whereas inhaled antigen results in a mixed airway and parenchymal response.