Hyaluronic acid inhibits the adherence and growth of monolayer keratinocytes but does not affect the growth of keratinocyte epithelium
Hyaluronic acid inhibits the adherence and growth of monolayer keratinocytes but does not affect the growth of keratinocyte epithelium
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DOI：
10.1007/s00403-006-0687-8
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发表时间：
2006-10-01
影响因子：
3
通讯作者：
Naukkarinen, Anita
中科院分区：
文献类型：
--
作者：
Harvima, Ilkka T.;Heikura, Hanna;Naukkarinen, Anita
透明质酸（HA）参与表皮生物学，但其功能意义的证据很少。在这项研究中，低钙单层和高钙上皮细胞培养的人角质形成细胞被用来研究多达四种不同的HA制剂对角质形成细胞的生长和增殖的角质形成细胞粘附到塑料表面上的不同基质蛋白质涂层的影响。在次优生长单层培养物中，高达1，000 μ g/ml的公鸡冠状HA和马链球菌HA抑制角质形成细胞生长。相反，使用最佳或次佳生长条件，测试的所有HA制剂不影响角质形成细胞上皮的生长和迁移。在细胞粘附试验中，高达1，000 μ g/ml的鸡冠HA和马链球菌HA可抑制角质形成细胞粘附到纤连蛋白和胶原蛋白包被的基质上。与其他HA制剂相比，来自人脐带的HA不影响单层角质形成细胞的生长，并且显著增加细胞粘附到胶原包被的基质上。然而，这种增加可归因于该HA制剂中存在的硫酸软骨素蛋白聚糖污染物。结论：HA能抑制增殖的单层角质形成细胞的生长和粘附，但对角质形成细胞上皮的生长和迁移无明显影响。
Hyaluronic acid (HA) is involved in epidermal biology but evidence for its functional significance is sparse. In this study, low-calcium monolayer and high-calcium epithelium cultures of human keratinocytes were used to study the effect of up to four different HA preparations on keratinocyte growth and on the adherence of proliferating keratinocytes onto the plastic surface coated with different matrix proteins. In suboptimally growing monolayer culture, up to 1,000 mu g/ml rooster comb HA and streptococcus equi HA inhibited keratinocyte growth. Instead, all HA preparations tested did not affect the growth and migration of keratinocyte epithelium using optimal or suboptimal growth conditions. In the cell adherence assays, up to 1,000 mu g/ml rooster comb HA and streptococcus equi HA inhibited the keratinocyte adherence onto the fibronectin- and collagen-coated substratum. In contrast to other HA preparations, HA from human umbilical cord did not affect the growth of monolayer keratinocytes and it increased markedly the cell adherence onto the collagen-coated substratum. This increase, however, can be attributed to chonroitin sulphate proteoglycan contaminant present in this HA preparation. In conclusion, HA can inhibit the growth and adherence of proliferating monolayer keratinocytes, but it has no apparent effect on the growth and migration of keratinocyte epithelium.