Wheat polyphenol oxidase: Distribution and genetic mapping in three inbred line populations
Wheat polyphenol oxidase: Distribution and genetic mapping in three inbred line populations
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DOI：
10.2135/cropsci2001.1750
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发表时间：
2001-11-01
期刊：
影响因子：
2.3
通讯作者：
Chang, HG
中科院分区：
文献类型：
--
作者：
Demeke, T;Morris, CF;Chang, HG
多酚氧化酶(PPO)与亚洲面条变色有关。以重组自交系M6/‘Opata 85’、NY18/CC和ND2603/‘Butte 86’为材料，研究了小麦(Triticum aestivum L.)PPO。用L-多巴(L-3.4-二羟基苯丙氨酸)和L-酪氨酸为底物测定PPO活性。M6/Opata 85 RIL群体的PPO活性服从正态分布，而ND2603/Butte 86 RIL群体的PPO活性呈双峰分布(L-DOPA法)。在所有三个种群中都观察到了越界分离。L-多巴和L-酪氨酸测定多酚氧化酶活性的相关性为低到中等，这可能归因于底物专一性和环境。在对M6/Opata 85重组自交系群体的联合分析中，QTL标记Xfba314(位于2D染色体上)与L-DOPA分析中的PPO活性显著相关。对于NY18/CC的联合分析，L-DOPA的三个QTL市场和L-酪氨酸的两个不同的QTL标记在>；2.4LOD值处与PPO活性有关。NY18/CC RIL群体的QTL标记位于第2A、2B、3D和6B染色体上。对ND2603/Butte 86群体进行连锁分析时，其他座位相对较少，仅有位于3BS染色体上的标记Xbcd907。L-DOPA分析表明，与PPO活性的关联较弱。已鉴定的QTL标记将有助于标记辅助选择，因为它们建立在这些群体的进化图谱上，并有助于更详细地解决小麦PPO活性的遗传基础。
The enzyme polyphenol oxidase (PPO) has been implicated in discoloration of Asian noodles. The recombinant inbred line (RIL) populations, M6/'Opata 85', NY18/CC, and ND2603/'Butte 86' were used to investigate the distribution, chromosome location, and number of loci involved in wheat (Triticum aestivum L.) PPO. PPO activity was measured by means of the substrates L-DOPA (L-3.4-dihydroxyphenyl-alanine) and L-tyrosine. The M6/Opata 85 RIL population had a normal distribution, while the ND2603/Butte 86 RIL population had a bimodal distribution for PPO activity (L-DOPA assay). Transgressive segregants were observed for all three populations. Correlations between L-DOPA and L.-tyrosine assays for PPO activity were low to medium and could be attributed to substrate specificity and environment. For the combined analysis of M6/Opata 85 RIL populations, the QTL marker Xfba314 (located on chromosome 2D) showed significant association with PPO activity for the L-DOPA assay. For the combined analysis of NY18/CC, three QTL markets for L-DOPA, and two different QTL markers for L-tyrosine, revealed an association with PPO activity at LOD scores of >2.4. The QTL markers for the NY18/CC RIL population were located on chromosomes 2A, 2B, 3D, and 6B. The ND2603/Butte 86 population had relatively few other loci for linkage analysis and only the marker Xbcd907.RV.I located on chromosome 3BS showed a weak association with PPO activity on the basis of the L-DOPA assay. The identified QTL markers will be useful for marker-assisted selection as they build upon the evolving maps for these populations, and for resolving in greater detail the genetic basis of PPO activity in wheat.