A new method for the quantitative analysis of endodontic microleakage
A new method for the quantitative analysis of endodontic microleakage
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DOI：
10.1016/s0099-2399(99)80136-8
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发表时间：
1999-03-01
影响因子：
4.2
通讯作者：
Allemann, C
中科院分区：
文献类型：
--
作者：
Haïkel, Y;Wittenmeyer, W;Allemann, C
本体外研究的目的是利用一种基于i -125放射性标记溶菌酶渗透定量分析的新方法，评估三种常用根管密封水泥（Sealapex、AH Plus或Topseal和Sealite）所获得的根尖密封效果。113颗牙的根管为直根管，达到根尖点25/30。这些被分为三组：(i)阴性对照组（4根）覆盖两层指甲油，（ii）试验组（105根）用三种胶合剂封闭横向凝聚的杜胶；阳性对照（4根）无骨水泥封闭。各组分别浸泡在I-125溶菌酶溶液中1、7、14或28天。切除后，用细金刚石丝将每根根切成6段，每段0.8 mm长。通过伽马计数器分析每个切片的活性，校正衰变，并用于量化蛋白质渗透。阳性对照中漏液量大，阴性对照中几乎可以忽略不计。随着时间的推移，AH Plus （Topseal）和Sealapex表现出类似的泄漏行为，AH Plus （Topseal）表现更好。直到第14天，Sealite的泄漏量还可以接受，之后泄漏量大幅增加，可能是由于三维不稳定造成的。
The aim of this in vitro study was to evaluate the apical seal obtained with three commonly used root canal sealing cements: Sealapex, AH Plus or Topseal, and Sealite, using a new method based on the quantitative analysis of I-125-radiolabeled lysozyme penetration. One hundred thirteen teeth with straight single root canals were instrumented to master apical point #25/30. These were divided into three groups: (i) negative control (4 roots) covered with two layers of nail polish, (ii) test group (105 roots) obturated by laterally condensed gutta-percha with the three cements; and (iii) positive control (4 roots) obturated without cement. The groups were then immersed in I-125 lysozyme solution for a period of 1, 7, 14, or 28 days. After removal, six sections of 0.8 mm length each were made of each root with a fine diamond wire. Each section was analyzed for activity by a gamma counter, corrected for decay, and used to quantify protein penetration. Leakage was high in the positive control and almost negligible in the negative control. AH Plus (Topseal) and Sealapex showed similar leakage behavior over time, with AH Plus (Topseal) performing better. Sealite showed acceptable leakage up until day 14, after which a large increase occurred, presumably due to three-dimensional instability.