Characterization of a Biofilm Membrane Reactor and Its Prospects for Fine Chemical Synthesis
Characterization of a Biofilm Membrane Reactor and Its Prospects for Fine Chemical Synthesis
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DOI：
10.1002/bit.22584
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发表时间：
2010-03-01
影响因子：
3.8
通讯作者：
Schmid, Andreas
中科院分区：
文献类型：
--
作者：
Gross, Rainer;Lang, Karsten;Schmid, Andreas
已知生物膜是稳健的生物催化剂。传统上，它们主要用于废水处理，然而最近关于它们用于化学合成的报道越来越引起人们的关注。利用在管式膜反应器中生长的工程假单胞菌菌株VLB 120 Delta C生物膜连续生产（S）-氧化苯乙烯。培养过程中，出水中出现了生物膜特异性形态型，无论接种条件如何，其均占总生物膜的60-80%，但其比活性与原始形态型相似。底物苯乙烯和产物氧化苯乙烯的传质被发现是依赖于流速，但不限制环氧化速率。氧气被确定为影响生物转化率的主要参数之一。生产率是线性依赖于比膜面积和管壁厚度。在没有反应器停机时间的情况下，在长的连续工艺周期（>= 50天）内已经实现了24 g L-aq（-1）天（-1）的平均体积生产率，最大值为70 g L-aq（-1）天（-1），以及45 gBDW L-aq（-1）的生物质浓度。Biotechnol. Bioeng. 2010;105：705-717. (C)2009 Wiley Periodicals，Inc.
Biofilms are known to be robust biocatalysts. Conventionally, they have been mainly applied for wastewater treatment, however recent reports about their employment for chemical synthesis are increasingly attracting attention. Engineered Pseudomonas sp. strain VLB120 Delta C biofilm growing in a tubular membrane reactor was utilized for the continuous production of (S)-styrene oxide. A biofilm specific morphotype appeared in the effluent during cultivation, accounting for 60-80% of the total biofilm irrespective of inoculation conditions but with similar specific activities as the original morphotype. Mass transfer of the substrate styrene and the product styrene oxide was found to be dependent on the flow rate but was not limiting the epoxidation rate. Oxygen was identified as one of the main parameters influencing the biotransformation rate. Productivity was linearly dependent on the specific membrane area and on the tube wall thickness. On average volumetric productivities of 24 g L-aq(-1) day(-1) with a maximum of 70 g L-aq(-1) day(-1) and biomass concentrations of 45 gBDW L-aq(-1) have been achieved over long continuous process periods (>= 50 days) without reactor downtimes. Biotechnol. Bioeng. 2010;105: 705-717. (C) 2009 Wiley Periodicals, Inc.