Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice
Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice
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DOI：
10.1093/biolre/iox150
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发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Handel, Mary Ann
中科院分区：
文献类型：
--
作者：
Hu, Jianjun;Sun, Fengyun;Handel, Mary Ann
真核翻译起始因子4G（EIF 4G）是翻译起始复合物中的重要支架蛋白。在小鼠中，Eif 4g 3基因突变导致雄性不育，在减数分裂前期结束时减数分裂停滞。本研究记录了EIF 4G 3的发育表达和亚细胞定位特征，这可能有助于其在减数分裂和精子发生中高度特异性的作用。非常出乎意料的是，EIF 4G 3位于精母细胞的细胞核中，在那里它高度富集在XY体中，XY体是由转录失活的性染色体形成的染色质结构域。此外，许多其他的，但不是所有的，翻译相关的蛋白质也定位在XY体.这些意想不到的意见牵连的XY体在控制mRNA代谢和/或“poising”蛋白质翻译复合物的减数分裂阶段前在spermocyte.Summary SentenceIn精母细胞，翻译起始因子EIF 4G 3本地化几乎完全的XY体，这表明在转录后调控精子发生基因表达的染色质结构域的作用。
Eukaryotic translation initiation factor 4G (EIF4G) is an important scaffold protein in the translation initiation complex. In mice, mutation of the Eif4g3 gene causes male infertility, with arrest of meiosis at the end of meiotic prophase. This study documents features of the developmental expression and subcellular localization of EIF4G3 thatmight contribute to its highly specific role in meiosis and spermatogenesis. Quite unexpectedly, EIF4G3 is located in the nucleus of spermatocytes, where it is highly enriched in the XY body, the chromatin domain formed by the transcriptionally inactive sex chromosomes. Moreover, many other, but not all, translation- related proteins are also localized in the XY body. These unanticipated observations implicate roles for the XY body in controlling mRNA metabolism and/or "poising" protein translation complexes before the meiotic division phase in spermatocytes.Summary SentenceIn spermatocytes, translation initiation factor EIF4G3 localizes almost exclusively to the XY body, suggesting a role for this chromatin domain in post-transcriptional regulation of spermatogenic gene expression.