Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress
Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress
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DOI：
10.1016/j.jplph.2013.10.017
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发表时间：
2014-03-01
影响因子：
4.3
通讯作者：
Ryslava, Helena
中科院分区：
文献类型：
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作者：
Hyskova, Veronika Doubnerova;Miedzinska, Lucia;Ryslava, Helena
干旱胁迫是最常见的非生物胁迫形式之一，它发生在水分有限的条件下。本研究探讨了磷酸烯醇式丙酮酸羧化酶（PEPC）、NADP-苹果酸酶（NADP-ME）和丙酮酸磷酸二激酶（PPDK）在烟草干旱胁迫中的作用。2.7.9.1 1.1.1.40 4.1.1.31 W38）进行了调查。干旱胁迫11天后，烟草叶片中的酶比活性显著增加，PEPC为对照的2.3倍，NADP-ME为对照的3.9倍，PPDK为对照的2.7倍。用定量RT PCR和免疫化学方法分别从转录水平和翻译水平研究了PEPC和NADP-ME活性的调节。叶绿体NADP-ME同工型的NADP-ME蛋白量和mRNA转录增加，表明它们的从头合成增强。另一方面，胞质NADP-ME亚型的mRNA减少。PEPC蛋白和PEPC mRNA的变化不明显。因此，评价了磷酸化对PEPC活性的调节，发现其参与应激反应。在恢复过程中，测试的酶的活动恢复到接近其基础水平。(C)2013年爱思唯尔有限公司。All rights reserved.
Drought stress is one of the most frequent forms of abiotic stresses, which occurs under condition of limited water availability. In this work, the possible participation of phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC), NADP-malic enzyme (EC 1.1.1.40; NADP-ME), and pyruvate, phosphate dikinase (EC 2.7.9.1; PPDK) in response to drought of tobacco plants (Nicotiana tabacum L, cv. W38) was investigated. Enzyme specific activities in tobacco leaves of drought stressed plants were significantly increased after 11 days of stress, PEPC 2.3-fold, NADP-ME 3.9-fold, and PPDK 2.7-fold compared to control plants. The regulation of PEPC and NADP-ME activities were studied on transcriptional level by the quantitative RT PCR and on translational level - immunochemically. The amount of NADP-ME protein and transcription of mRNA for chloroplastic NADP-ME isoform were increased indicating their enhanced synthesis de novo. On the other hand, mRNA for cytosolic isoform of NADP-ME was decreased. The changes in PEPC protein and PEPC mRNA were not substantial. Therefore regulation of PEPC activity by phosphorylation was evaluated and found to be involved in the stress response. During recovery, activities of the tested enzymes returned close to their basal levels. (C) 2013 Elsevier GmbH. All rights reserved.