Chemical Synthesis of Homogeneous Glycopeptides and Glycoproteins
Chemical Synthesis of Homogeneous Glycopeptides and Glycoproteins
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DOI：
10.1002/tcr.200900024
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发表时间：
2010-01-01
期刊：
影响因子：
6.6
通讯作者：
Murase, Takefumi
中科院分区：
文献类型：
--
作者：
Kajihara, Yasuhiro;Yamamoto, Naoki;Murase, Takefumi
已知与蛋白质连接的寡核苷酸在几种生物事件中发挥重要作用。然而，寡糖是异质的，这阻碍了寡糖功能的详细阐明。为了解决这个问题，长期以来一直需要具有均质寡糖的糖蛋白。为此，研究了一种从天然资源中有效制备复合型寡糖的方法，发现该方法通过使用化学方法和分支特异性连续糖苷酶消化来提供超过24种不同的复合型寡糖。获得的足够量的均质复合型寡糖使我们能够通过使用固相糖肽合成方法和天然化学连接来检查均质糖肽以及糖蛋白的合成。本文综述了近年来寡糖的高效制备方法和包括促红细胞生成素在内的糖蛋白的合成研究进展。(C)2010年日本化学杂志论坛和Wiley Periodicals，Inc. Chem Rec 10：80-100; 2010：在线发表于Wiley InterScience（www.interscience.wiley.com）DOI 10.1002/tcr.200900024
Oligosaccharides linked to proteins are known to play important roles in several biological events. However, oligosaccharides are heterogeneous, which has hindered detailed elucidation of oligosaccharide functions. In order to solve this problem, glycoproteins having homogeneous oligosaccharides have long been required. For this purpose, an efficient preparative method of complex-type oligosaccharides has been investigated from a natural source and this method was found to afford over 24 kinds of diverse complex-type oligosaccharides by use of chemical methods and branch-specific sequential glycosidase digestion. The sufficient amount of homogeneous complex type oligosaccharides obtained enabled us to examine the synthesis of homogeneous glycopeptides as well as glycoproteins by use of solid phase glycopeptide synthetic method and native chemical ligation. This review describes recent progress related to the efficient method of oligosaccharide preparation and synthesis of glycoproteins including bioactive erythropoietin. (C) 2010 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 10: 80-100; 2010: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.200900024