Pathways of electron transfer in Escherichia coli DNA photolyase: Trp306 to FADH.

Pathways of electron transfer in Escherichia coli DNA photolyase: Trp306 to FADH.
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大肠杆菌 DNA 光裂合酶中的电子转移途径:Trp306 至 FADH。

DOI:
10.1016/s0006-3495(99)77287-5
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发表时间:
1999
影响因子:
3.4
通讯作者:
Heelis,PF
Heelis,PF
中科院分区:
生物学3区
文献类型:
--
作者:
Cheung,MS;Daizadeh,I;Stuchebrukhov,AA;Heelis,PF

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我们描述了使用原子间隧道电流方法对大肠杆菌DNA光裂合酶分子中Trp 306和 *FADH·之间的电子转移途径进行的一系列理论计算的结果。结果表明,在电子传递过程中,供体和受体之间存在两个构象正交的双链体Trp359和Trp382。这些途径主要取决于甘草酸和黄素分子的芳香性。计算结果表明,电子转移的主要途径是一组相互重叠的正交π环,从供体Trp306开始,经过Trp359和Trp382,最后到达受体络合物FADH的黄素基团。
We describe the results of a series of theoretical calculations of electron transfer pathways between Trp306and *FADH·in theEscherichia coliDNA photolyase molecule, using the method of interatomic tunneling currents. It is found that there are two conformationally orthogonal tryptophans, Trp359and Trp382, between donor and acceptor that play a crucial role in the pathways of the electron transfer process. The pathways depend vitally on the aromaticity of tryptophans and the flavin molecule. The results of this calculation suggest that the major pathway of the electron transfer is due to a set of overlapping orthogonalπ-rings, which starts from the donor Trp306, runs through Trp359and Trp382, and finally reaches the flavin group of the acceptor complex, FADH.
DOI: 10.1126/science.7604260
发表时间: 1995-06-30
期刊: SCIENCE
影响因子: 56.9
作者:
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发表时间: 1997-04-18
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发表时间: 1997-04-15
影响因子: 11.1
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