Ganglioside-induced adherence of botulinum and tetanus neurotoxins to adducin.
Ganglioside-induced adherence of botulinum and tetanus neurotoxins to adducin.
复制标题
神经节苷脂诱导肉毒杆菌和破伤风神经毒素粘附到内收蛋白。
DOI：
10.1046/j.1471-4159.1996.66062556.x
复制
发表时间：
1996
影响因子：
4.7
通讯作者：
Ringler,NJ
中科院分区：
文献类型：
--
作者：
Schengrund,CL;DasGupta,BR;Hughes,CA;Ringler,NJ
先前发现肉毒毒素血清型A、B或E与神经节苷脂GT1b预孵育可增强肉毒毒素与突触素I和~ 116‐kDa牛脑突触体蛋白的粘附性；相反，破伤风神经毒素对这两种蛋白的粘附需要与GT1b进行预孵育。我们现在已经发现，与神经节苷脂GD3一起预孵育的神经毒素比GT1b更能增强它们对~ 116‐kDa蛋白的粘附性。通过制备柱十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和二维凝胶电泳，从牛脑突触体中获得了纯化的水溶性~ 116 - kDa蛋白。获得了~ 116‐kDa蛋白的两个色氨酸片段的N端氨基酸序列。这些序列与细胞骨架蛋白β -内收蛋白数据库序列相匹配。神经毒素粘附于内收蛋白的羧基末端尾部区域，而非头部区域。神经毒素粘附于内收蛋白和突触蛋白I可能促进神经毒素向其特定底物的呈递。
Preincubation of botulinum neurotoxin serotype A, B, or E with ganglioside GT1b was previously found to enhance adherence of botulinum neurotoxin to synapsin I and an ∼116‐kDa bovine brain synaptosomal protein; in contrast, adherence to these two proteins by tetanus neurotoxin required preincubation with GT1b. We have now found that preincubation of the neurotoxins with ganglioside GD3 enhances their adherence to the ∼116‐kDa protein more than that with GT1b. A purified preparation of the water‐soluble ∼116‐kDa protein was obtained from bovine brain synaptosomes by preparative column sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and two‐dimensional gel electrophoresis. N‐Terminal amino acid sequences were obtained for two tryptic fragments of the ∼116‐kDa protein. These sequences matched with the data bank sequences for β‐adducin, a cytoskeletal protein. The carboxy‐terminal tail region of adducin, but not the head region, was adhered to by the neurotoxins. Adherence of the neurotoxin to adducin and synapsin I may facilitate presentation of the neurotoxin to its specific substrate(s).