Molecular Pathology in Vulnerable Carotid Plaques: Correlation with [18]-Fluorodeoxyglucose Positron Emission Tomography (FDG-PET)
Molecular Pathology in Vulnerable Carotid Plaques: Correlation with [18]-Fluorodeoxyglucose Positron Emission Tomography (FDG-PET)
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DOI：
10.1016/j.ejvs.2008.11.018
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发表时间：
2009-06-01
影响因子：
5.7
通讯作者：
Kjaer, A.
中科院分区：
文献类型：
--
作者：
Graebe, M.;Pedersen, S. F.;Kjaer, A.
目的：动脉粥样硬化被认为是一种炎症性疾病，新的诊断工具是必要的，以评估斑块炎症活动和心血管事件的风险。我们研究了通过正电子发射断层扫描（PET）可视化的脆弱颈动脉斑块中[18]-氟脱氧葡萄糖（FDG）的吸收。摄取与炎症和斑块易损性的已知标记物的定量基因表达相关。方法：10例近期短暂性脑缺血发作和颈动脉狭窄（>50%）的患者在颈动脉内膜切除术前一天进行了FDG-PET和计算机断层扫描血管造影（CTA）。使用实时定量聚合酶链反应定量炎症细胞因子白细胞介素18（IL-18）、巨噬细胞特异性标志物CD 68和两种蛋白酶组织蛋白酶K和基质金属蛋白酶9（MMP-9）的斑块mRNA表达。与参考动脉标本相比，在斑块中发现了组织蛋白酶K（2.1倍+/-0.5）、MMP-9（122倍+/-65）和IL-18（3.4倍+/-0.7）。斑块摄取FDG与CD 68基因表达呈显著正相关（r = 0.71，P = 0.02）。任何相关性与组织蛋白酶K，MMP-9或IL-18基因expression were weaken.Conclusions：FDG-PET摄取在颈动脉斑块的CD 68和其他分子标志物的炎症和脆弱性的基因表达相关。(C)2008年欧洲血管外科学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: Atherosclerosis is recognised as an inflammatory disease, and new diagnostic tools are warranted to evaluate plaque inflammatory activity and risk of cardiovescular events. We investigated [18]-fluorodeoxyglucose (FDG) uptake in vulnerable carotid plaques visualised by positron emission tomography (PET). Uptake was correlated to quantitative gene expression of known markers of inflammation and plaque vulnerability.Methods: Ten patients with recent transient ischaemic attack and carotid artery stenosis (>50%) underwent combined FDG-PET and computed tomography angiography (CTA) the day before carotid endarterectomy. Plaque mRNA expression of the inflammatory cytokine interleukin 18 (IL-18), the macrophage-specific marker CD68 and the two proteinases, Cathepsin K and matrix metalloproteinase 9 (MMP-9), were quantified using real-time quantitative polymerase chain reaction.Results: Consistent up-regulation of CD68 (3.8-fold +/- 0.9; mean +/- standard error), Cathepsin K (2.1-fold +/- 0.5), MMP-9 (122-fold +/- 65) and IL-18 (3.4-fold +/- 0.7) were found in the plaques, compared to reference-artery specimens. The FDG uptake by plaques was strongly correlated with CD68 gene expression (r = 0.71, P = 0.02). Any correlations with Cathepsin K, MMP-9 or IL-18 gene expression were weaker.Conclusions: FDG-PET uptake in carotid plaques is correlated to gene expression of CD68 and other molecular markers of inflammation and vulnerability. (C) 2008 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.