The mechanism of cell differentiation in Bacillus subtilis
The mechanism of cell differentiation in Bacillus subtilis
复制标题
DOI：
10.1038/nature04666
复制
发表时间：
2006-05-18
期刊：
影响因子：
64.8
通讯作者：
Campbell, ID
中科院分区：
文献类型：
--
作者：
Iber, D;Clarkson, J;Campbell, ID
枯草芽孢杆菌中的孢子形成可作为从单个细胞发育成两种不同细胞类型的模型(1)。尽管已经积累了大量关于启动发育计划的机制的信息(2)，但仍然存在只能通过定量分析才能回答的重要问题。在这里，我们借助现有和新的实验结果开发了一个数学模型，该模型重现已发表的体外实验，并解释了关键转录因子的激活是如何调节的。该模型将两种细胞类型之间的体积差异确定为决定细胞命运的主要触发因素。它表明这种效应取决于关键蛋白激酶的变构行为以及相应磷酸酶的低去磷酸化率；这两种预测的效果都得到了实验的证实。
Sporulation in Bacillus subtilis serves as a model for the development of two different cell types from a single cell(1). Although much information has been accumulated about the mechanisms that initiate the developmental programmes(2), important questions remain that can be answered only by quantitative analysis. Here we develop, with the help of existing and new experimental results, a mathematical model that reproduces published in vitro experiments and explains how the activation of the key transcription factor is regulated. The model identifies the difference in volume between the two cell types as the primary trigger for determining cell fate. It shows that this effect depends on the allosteric behaviour of a key protein kinase and on a low rate of dephosphorylation by the corresponding phosphatase; both predicted effects are confirmed experimentally.