Differential growth factor content of uterine luminal fluids from large white and prolific Meishan pigs during the estrous cycle and early pregnancy.
Differential growth factor content of uterine luminal fluids from large white and prolific Meishan pigs during the estrous cycle and early pregnancy.
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DOI：
10.2527/jas1989.6761538x
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发表时间：
1989-06
影响因子：
3.3
通讯作者：
R. Simmen;F. Simmen;Y. Ko;F. Bazer
中科院分区：
文献类型：
--
作者：
R. Simmen;F. Simmen;Y. Ko;F. Bazer
比较了大白色（LW）和多产中国梅山（MS）后备母猪在发情周期和妊娠早期子宫腔液（ULF）中肽生长因子的含量。胰岛素样生长因子-I（IGF-I）通过RIA定量;子宫腔液有丝分裂原（ULFM）的体外促生长特性通过[3 H]胸苷掺入培养的静止AKR-2B成纤维细胞的DNA来测量。峰浓度大白色母猪和梅山母猪ULF中IGF-Ⅰ的含量（pg/μ g ULF蛋白）分别为：发情周期9.8 ± 1.4（第10天）和39.7 ± 7.8（第12天）;妊娠期，13.1 ± 3.2（第8天和第10天）和11.9 ± 2.1（第12天），天数间差异（第10天除外，P>.5）受品种影响（P <.10）。对于这两个品种，有一个快速下降的IGF-I浓度的第14天的周期和怀孕。在发情周期和妊娠期的第10 - 14天，LW母猪的子宫腔液有丝分裂原活性大于MS母猪（P <0.01），并且在两个品种中均以时间依赖性方式减少。两个品种的IGF-I浓度和子宫重量之间没有相关性。相比之下，在周期（MS：r = -.855，P小于.10; LW：r = -.834，P小于.05）和妊娠（MS：r = -.806，P小于.10; LW：r = -.928，P小于.05）后备母猪中检测到子宫重量与ULFM活性之间呈负相关。（250字处删节）
Uterine luminal fluids (ULF) from Large White (LW) and prolific Chinese Meishan (MS) gilts were compared with respect to their peptide growth factor content during an estrous cycle and early pregnancy. Insulin-like growth factor-I (IGF-I) was quantitated by RIA; in vitro growth promoting properties of uterine luminal fluid mitogen (ULFM) were measured by [3H]thymidine incorporation into DNA of quiescent AKR-2B fibroblastic cells in culture. Peak concentrations (pg/microgram ULF protein) of IGF-I in ULF of Large White and Meishan gilts, respectively, were: estrous cycle, 9.8 +/- 1.4 (on d 10) and 39.7 +/- 7.8 (on d 12); gestation, 13.1 +/- 3.2 (on d 8 and 10) and 11.9 +/- 2.1 (on d 12), with differences among days (except d 10, P greater than .5) being affected by breed (P less than .10). For both breeds, there was a rapid decline in IGF-I concentrations by d 14 of the cycle and of pregnancy. Uterine luminal fluid mitogen activity was greater (P less than .01) for LW than for MS gilts on d 10 to 14 of an estrous cycle and gestation and diminished in a time-dependent manner in both breeds. No correlation was observed between IGF-I concentrations and uterine weights for either breed. In contrast, a negative correlation between uterine weight and ULFM activity was detected for cyclic (MS: r = -.855, P less than .10; LW: r = -.834, P less than .05) and pregnant (MS: r = -.806, P less than .10; LW: r = -.928, P less than .05) gilts.(ABSTRACT TRUNCATED AT 250 WORDS)