Classification and fingerprinting of different berries based on biochemical profiling and antioxidant capacity
Classification and fingerprinting of different berries based on biochemical profiling and antioxidant capacity
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DOI：
10.1590/s0100-204x2013000900013
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发表时间：
2013-09-01
期刊：
影响因子：
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通讯作者：
Maksimović, Vuk
中科院分区：
文献类型：
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作者：
Milivojević, Jasminka;Rakonjac, Vera;Maksimović, Vuk
本工作的目的是评估属于草莓属，悬钩子属，越橘属和茶藨子属的6个浆果类型的生化成分。水果样品收集了一式三份（50个水果），从18个不同的物种或品种的上述属，在三年（2008年至2010年）。采用高效液相色谱法（HPLC）测定了各糖、有机酸、黄酮醇和酚酸的含量，采用分光光度法测定了总酚（TPC）和总抗氧化能力（TAC）。主成分分析（PCA）和系统聚类分析（CA）进行评价果实生化特性的差异。黑穗醋栗、野草莓、褶纹悬钩子和欧洲越橘中的活性成分含量最高。PCA和CA能够部分区分浆果的生化成分的基础上。单个糖和总糖、杨梅素、鞣花酸、TPC和TAC对浆果的生化组成影响最大。CA分离黑莓，乌藨子，蓝莓作为隔离组，而分类草莓，黑醋栗和红醋栗在一个特定的组还没有发生。不同类型的浆果之间和内部都有很大的差异。评价浆果的代谢物指纹图谱显示独特的生化概况和生物活性化合物含量的特定组合。
The objective of this work was to evaluate the biochemical composition of six berry types belonging to Fragaria, Rubus, Vaccinium and Ribes genus. Fruit samples were collected in triplicate (50 fruit each) from 18 different species or cultivars of the mentioned genera, during three years (2008 to 2010). Content of individual sugars, organic acids, flavonols, and phenolic acids were determined by high performance liquid chromatography (HPLC) analysis, while total phenolics (TPC) and total antioxidant capacity (TAC), by using spectrophotometry. Principal component analysis (PCA) and hierarchical cluster analysis (CA) were performed to evaluate the differences in fruit biochemical profile. The highest contents of bioactive components were found in Ribes nigrum and in Fragaria vesca, Rubus plicatus, and Vaccinium myrtillus. PCA and CA were able to partially discriminate between berries on the basis of their biochemical composition. Individual and total sugars, myricetin, ellagic acid, TPC and TAC showed the highest impact on biochemical composition of the berry fruits. CA separated blackberry, raspberry, and blueberry as isolate groups, while classification of strawberry, black and red currant in a specific group has not occurred. There is a large variability both between and within the different types of berries. Metabolite fingerprinting of the evaluated berries showed unique biochemical profiles and specific combination of bioactive compound contents.