SIALIC-ACID CONTENTS IN CHICKEN EGGS AND TISSUES
SIALIC-ACID CONTENTS IN CHICKEN EGGS AND TISSUES
复制标题
DOI：
10.4141/cjas94-088
复制
发表时间：
1994-12-01
影响因子：
1
通讯作者：
SIM, JS
中科院分区：
文献类型：
--
作者：
NAKANO, K;NAKANO, T;SIM, JS
唾液酸含量被确定为潜在的商业来源，在鸡蛋部分（蛋黄，白色，合点，蛋黄膜和壳膜），输卵管，公鸡冠和肉垂组织的高碘酸盐-硫代巴比妥酸法与部分修改。在卵组分中，唾液酸含量最高（P < 0.05）（13.7 +/- 0.5 μ g mg（-1）），较高合点区（P < 0.05）（5.1 +/- 0.2 μ g mg（-1））和蛋黄（5.2 +/- 0.4 μ g mg（-1））比在白色中（2.2 +/- 0.3 μ g mg（-1））低，并且在壳膜中最低（P < 0.05）（0.6 +/- 0.1 μ g mg（-1））。在输卵管中，Magnum中的唾液酸浓度（3.7 +/- 0.6 pg mg（-1））低于（P < 0.05）其余区域，其中浓度相似（5.6 +/- 0.3 μ g mg（-1））。梳状组织和荆条组织中的唾液酸浓度相似（2.6 +/- 0.4 μ g mg（-1））。用木瓜蛋白酶水解和凝胶层析法从蛋黄、白色、鸡冠和荆肉组织中分离出含唾液酸的糖肽组分。
Sialic acid contents were determined as potential commercial sources in egg fractions (yolk, white, chalaza, yolk membrane and shell membrane), oviduct, and rooster comb and wattle tissues by the periodate-thiobarbituric acid method with a partial modification. In egg fractions, the concentration of sialic acid was highest (P < 0.05) in yolk membrane (13.7 +/- 0.5 mu g mg(-1)), higher (P < 0.05) in chalaza (5.1 +/- 0.2 mu g mg(-1)) and yolk (5.2 +/- 0.4 mu g mg(-1)) than in white (2.2 +/- 0.3 mu g mg(-1)), and lowest (P < 0.05) in shell membrane (0.6 +/- 0.1 mu g mg(-1)). In oviduct, the concentration of sialic acid was lower (P < 0.05) in magnum (3.7 +/- 0.6 pg mg(-1)) than in the remaining regions, among which the concentration was similar (5.6 +/- 0.3 mu g mg(-1)). The sialic acid concentration was similar in comb and wattle tissues (2.6 +/- 0.4 mu g mg(-1)). Sialic acid containing glycopeptide fractions were isolated from yolk, white, and comb and wattle tissues by papain proteolysis and gel chromatography.