Fourier transform infrared imaging spectroscopic analysis of tissue engineered cartilage: histologic and biochemical correlations
Fourier transform infrared imaging spectroscopic analysis of tissue engineered cartilage: histologic and biochemical correlations
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DOI：
10.1117/1.1922329
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发表时间：
2005-05-01
影响因子：
3.5
通讯作者：
Camacho, NP
中科院分区：
文献类型：
--
作者：
Kim, M;Bi, XH;Camacho, NP
软骨的组成可预测其体内性能。因此，评估其主要大分子组分蛋白聚糖（PG）和胶原蛋白的能力非常重要。在目前的研究中，我们假设PG的含量和组织工程软骨中的分布可以确定使用傅里叶变换红外成像光谱（FT-IRIS）。软骨是从软骨细胞中生长的中空纤维生物反应器（HFBR）系统，以前广泛用于研究软骨发育。FT-IRIS分析表明，PG含量的梯度，与中心附近的营养纤维和最低的HFBR的内表面附近的含量最高。此外，我们发现，与流出区（24.7%）相比，靠近培养基流入区（45.0%）的PG含量显著更高（p < 0.001）。该差异抵消了生化测定的糖胺聚糖差异42.6%与27.8%。此外，组织切片内特定位置的FT-IRIS测定的PG含量与组织学测定的PG含量相关（R=50.73，p=50.007）。总之，PG的FT-IRIS测定与PG的组织学测定相关，并产生与发育中软骨中糖胺聚糖的生化测定定量相似的结果。(c)2005年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determined using Fourier-transform infrared imaging spectroscopy (FT-IRIS). The cartilage was grown from chondrocytes within a hollow fiber bioreactor (HFBR) system previously used extensively to study cartilage development. FT-IRIS analysis showed a gradient of PG content, with the highest content in the center near the nutritive fibers and the lowest near the interior surface of the HFBR. Further, we found significantly greater PG content in the region near culture medium inflow (45.0%) as compared to the outflow region (24.7%) (p < 0.001). This difference paralleled the biochemically determined glycosaminoglycan difference of 42.6% versus 27.8%. In addition, FT-IRIS-determined PG content at specific positions within the tissue sections correlated with histologically determined PG content (R=50.73, p=50.007). In summary, FT-IRIS determination of PG correlates with histological determination of PG and yields quantitatively similar results to biochemical determination of glycosaminoglycan in developing cartilage. (c) 2005 Society of Photo-Optical Instrumentation Engineers.