The function of intermediate filaments in cell shape and cytoskeletal integrity.
The function of intermediate filaments in cell shape and cytoskeletal integrity.
复制标题
DOI：
10.1083/jcb.134.4.971
复制
发表时间：
1996-08
期刊：
影响因子：
--
通讯作者：
Steinert PM
中科院分区：
文献类型：
--
作者：
Goldman RD;Khuon S;Chou YH;Opal P;Steinert PM
本研究描述了一种特定方法的开发和使用，用于拆卸活细胞中的中间丝（IF）网络。它利用了来自IF蛋白链的螺旋起始1A结构域的氨基酸序列的模拟肽的破坏性作用。结果表明，在1：1的摩尔比，这些肽在体外室温下在30分钟内将波形蛋白IF分解成小的寡聚复合物和单体。显微注射到培养的成纤维细胞后，这些相同的肽诱导IF网络的快速解体。解体过程伴随着细胞形状的急剧变化以及微管和肌动蛋白-应力纤维网络的不稳定。细胞形状和IF组装状态的这些变化是可逆的。讨论了IF在细胞形状和维持细胞质的完整性和机械性能，以及其他主要细胞骨架系统的稳定性方面的作用的结果。
This study describes the development and use of a specific method for disassembling intermediate filament (IF) networks in living cells. It takes advantage of the disruptive effects of mimetic peptides derived from the amino acid sequence of the helix initiation 1A domain of IF protein chains. The results demonstrate that at 1:1 molar ratios, these peptides disassemble vimentin IF into small oligomeric complexes and monomers within 30 min at room temperature in vitro. Upon microinjection into cultured fibroblasts, these same peptides induce the rapid disassembly of IF networks. The disassembly process is accompanied by a dramatic alteration in cell shape and the destabilization of microtubule and actin-stress fiber networks. These changes in cell shape and IF assembly states are reversible. The results are discussed with respect to the roles of IF in cell shape and the maintenance of the integrity and mechanical properties of the cytoplasm, as well as the stability of the other major cytoskeletal systems.