Tumor necrosis factor-alpha in gestation and puerperium of women with gestational hypertension and pre-eclampsia
Tumor necrosis factor-alpha in gestation and puerperium of women with gestational hypertension and pre-eclampsia
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DOI：
10.1111/j.1600-0897.2006.00455.x
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Serrao Peracoli, Maria Terezinha
中科院分区：
文献类型：
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作者：
Peracoli, Jose Carlos;Cunha Rudge, Marilza Vieira;Serrao Peracoli, Maria Terezinha
孕妇血浆中肿瘤坏死因子- α (tnf - α)的高水平与子痫前期（PE）的发病机制有关。本研究评估了妊娠期和产褥期妊娠高血压（GH）和妊娠期PE患者的tnf - α血浆水平和单核细胞生成。研究方法本研究纳入128名妇女，其中20名为非妊娠期（NP）血压正常者（NT）， 108名为妊娠期（NT）， 36名为妊娠期，27名GH， 45名PE。外周血血浆用于tnf - α和尿酸测定。采用L929生物测定法测定血浆和脂多糖刺激和非刺激单核细胞上清液中tnf - α的含量。结果PE组血浆中肿瘤坏死因子α和尿酸水平高于GH组。在两组高血压患者中，这些参数均呈正相关，且明显高于NT组和NP组。妊娠期高血压妇女的tnf - α血浆水平和单核细胞生成高于妊娠期NT妇女，产褥期显著降低。尽管lps刺激的PE单核细胞中tnf - α释放减少，但仍显著高于其他妊娠组。结论GH和PE孕妇体内单核细胞活化以体外tnf - α生成为特征。在PE中观察到的tnf - α和尿酸的循环浓度高于GH，这表明PE与疾病严重程度有关。
Problem High plasma levels of tumor necrosis factor-alpha (TNF-alpha) in pregnant women have been associated with the pathogenesis of pre-eclampsia (PE). This study evaluated TNF-alpha plasma levels and monocyte production in gestational hypertension (GH) and PE during gestation and at puerperium.Method of study This study included 128 women, of whom 20 were non-pregnant (NP) normotensive (NT), and 108 were pregnant: 36 NT, 27 with GH, and 45 with PE. Peripheral blood plasma was used for TNF-alpha and uric acid determination. TNF-alpha was determined in plasma and lipopolysaccharide (LPS)-stimulated and non-stimulated monocyte supernatants by L929 bioassay.Results Tumor necrosis factor-alpha and uric acid plasma levels were higher in PE than in GH pregnancies. In both hypertensive groups, these parameters positively correlated and were significantly more elevated than in NT and NP women. TNF-alpha plasma levels and monocyte production were higher in hypertensive than in NT women during gestation, and significantly decreased at puerperium. Although decreased, TNF-alpha release in LPS-stimulated PE monocytes, was still significantly higher than in the other pregnant groups.Conclusion In vivo monocyte activation in GH and PE pregnant women was characterized by in vitro TNF-alpha production. The fact that higher circulating concentrations of TNF-alpha and uric acid were observed in PE than in GH suggests an association with disease severity.