Developing cold-adapted biomass for the anaerobic treatment of domestic wastewater at low temperatures (4, 8 and 15 °C) with inocula from cold environments.
Developing cold-adapted biomass for the anaerobic treatment of domestic wastewater at low temperatures (4, 8 and 15 °C) with inocula from cold environments.
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DOI：
10.1016/j.watres.2016.12.009
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发表时间：
2017-04
期刊：
影响因子：
12.8
通讯作者：
E. Petropoulos;J. Dolfing;R. Davenport;Emma J. Bowen;T. Curtis
中科院分区：
文献类型：
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作者：
E. Petropoulos;J. Dolfing;R. Davenport;Emma J. Bowen;T. Curtis
温度是温带地区生活污水厌氧处理的瓶颈。以前大多数在低温下实现厌氧处理的尝试都试图驯化中温污泥，但在低于10-13摄氏度的温度下失败了。我们描述了一种替代方法，使用在进化时间尺度上暴露于低温环境中的群落作为此类反应器的种子。在间歇反应器中接种来自北极高原和阿尔卑斯湖的土壤和沉积物的混合物，在4℃、8℃和15℃下处理紫外线灭菌的原始生活污水。为评价细菌的内在处理能力，评价了产甲烷和水解率。在4℃、8℃和15℃下的比产甲烷活性分别为6.3fmolCH4cell−1day−1和10.3fmolCH4cell。特定的水解率分别为76.2、186.6和251.9 fg COD cell−1day−1。水解率是产甲烷的温度敏感度的两倍(Q10：4.6 2和1.5 7)。这些比速率比我们以前在微观宇宙中观察到的高出十倍以上，这些微宇宙以相同温度下的沉淀废水喂养。结果表明，接种冷适应群落的反应器是一种很有前途的方法，可以开发出能够在低温下处理厌氧废水的生物质，同时实现符合EC指令COD标准的出水。如果能达到令人满意的细胞浓度，大规模的反应器是可行的。
Temperature is the bottleneck for the anaerobic treatment of domestic wastewater in temperate climates. Most previous attempts to achieve anaerobic treatment at low temperatures have attempted to acclimatize mesophilic sludge and have failed at temperatures below 10–13 °C. We describe an alternative approach using communities from environments that have been exposed to low temperatures over evolutionary time-scales as seed for such reactors. Batch reactors were inoculated with a mixture of soils and sediments from the high Arctic and an Alpine lake to treat UV-sterilized raw domestic wastewater at 4, 8 and 15 °C. To evaluate the intrinsic treatment capacity of the bacteria the specific rates of methanogenesis and hydrolysis were evaluated. Specific methanogenic activity at 4, 8 and 15 °C was 6.3, 7.6 and 10.3 fmol CH4cell−1day−1respectively. Specific putative hydrolysis rates were 76.2, 186.6 and 251.9 fgrams COD cell−1day−1. Hydrolysis was twice as temperature sensitive as methanogenesis (Q10: 4.62 and 1.57 respectively). The specific rates are over ten times higher than we have previously observed in microcosms fed with settled wastewater at the same temperatures. The results imply that inoculating reactors with cold-adapted communities is a promising way to develop biomass capable of treating anaerobic wastewater treatment at low temperatures whilst achieving an effluent that conforms to the EC Directive COD standards. Large-scale reactors are feasible if satisfactory cell concentrations can be achieved.