Wide-field, high-resolution lensless on-chip microscopy via near-field blind ptychographic modulation
Wide-field, high-resolution lensless on-chip microscopy via near-field blind ptychographic modulation
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DOI：
10.1039/c9lc01027k
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发表时间：
2020-03-21
期刊：
影响因子：
6.1
通讯作者：
Zheng, Guoan
中科院分区：
文献类型：
--
作者：
Jiang, Shaowei;Zhu, Jiakai;Zheng, Guoan
提出了一种基于近场盲重叠关联调制的无透镜片上显微平台。在这个平台中，我们在物体和图像传感器之间放置了一个薄的漫射器，用于光波调制。通过盲扫描未知漫射器到不同的x-y位置，我们获得了一系列调制强度图像，用于定量对象恢复。与以前的重叠关联实现不同，我们采用了单位放大率配置与菲涅耳数类似的50000，这是数量级高于以前的重叠关联设置。单位放大率配置允许我们将整个传感器区域（6.4 mm × 4.6 mm）作为成像视场。超高的菲涅尔数使我们能够直接恢复的相位恢复过程中的扩散器的位置偏移，解决定位精度问题，定期重叠关联实验。在我们的实现中，我们使用一个低成本的，DIY的扫描阶段，以执行盲漫射器调制。精确的机械扫描，这是传统的重叠关联实验中的关键是不再需要在我们的设置。我们进一步采用了上采样相位恢复方案，以绕过成像器像素大小设置的分辨率限制，并展示了0.78 μ m的半间距分辨率。我们通过体外细胞培养、透明和染色的组织切片以及厚的生物样品来验证成像性能。我们发现，恢复的定量相位图可以用来执行有效的细胞分割的密集的酵母培养。我们还展示了基于恢复的波前的厚生物样品的3D数字重聚焦。该平台为大视场、高分辨率和定量的片上显微镜提供了一种具有成本效益的交钥匙解决方案。它适用于广泛的护理点，全球健康和远程医疗相关应用。
We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning the unknown diffuser to different x-y positions, we acquire a sequence of modulated intensity images for quantitative object recovery. Different from previous ptychographic implementations, we employ a unit magnification configuration with a Fresnel number of similar to 50000, which is orders of magnitude higher than those of previous ptychographic setups. The unit magnification configuration allows us to have the entire sensor area, 6.4 mm by 4.6 mm, as the imaging field of view. The ultra-high Fresnel number enables us to directly recover the positional shift of the diffuser in the phase retrieval process, addressing the positioning accuracy issue plaguing regular ptychographic experiments. In our implementation, we use a low-cost, DIY scanning stage to perform blind diffuser modulation. Precise mechanical scanning that is critical in conventional ptychography experiments is no longer needed in our setup. We further employ an up-sampling phase retrieval scheme to bypass the resolution limit set by the imager pixel size and demonstrate a half-pitch resolution of 0.78 mu m. We validate the imaging performance via in vitro cell cultures, transparent and stained tissue sections, and a thick biological sample. We show that the recovered quantitative phase map can be used to perform effective cell segmentation of a dense yeast culture. We also demonstrate 3D digital refocusing of the thick biological sample based on the recovered wavefront. The reported platform provides a cost-effective and turnkey solution for large field-of-view, high-resolution, and quantitative on-chip microscopy. It is adaptable for a wide range of point-of-care-, global-health-, and telemedicine-related applications.