A mammalian oocyte-specific linker histone gene H1oo: homology with the genes for the oocyte-specific cleavage stage histone (cs-H1) of sea urchin and the B4/H1M histone of the frog.
A mammalian oocyte-specific linker histone gene H1oo: homology with the genes for the oocyte-specific cleavage stage histone (cs-H1) of sea urchin and the B4/H1M histone of the frog.
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发表时间：
2001-03
期刊：
影响因子：
4.6
通讯作者：
Mamoru Tanaka;Jon D. Hennebold;Jane L. Macfarlane;Eli Y. Adashi
中科院分区：
文献类型：
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作者：
Mamoru Tanaka;Jon D. Hennebold;Jane L. Macfarlane;Eli Y. Adashi
多个（非哺乳动物）物种的卵母细胞和早期胚胎缺乏连接体组蛋白H1的体细胞形式。据我们所知，哺乳动物卵母细胞特异性接头（H1）组蛋白尚未报道。我们在差异筛选（抑制消减杂交（SSH））项目的过程中发现了有问题的cDNA。该新的1.2 kb cDNA的全长序列的解析导致了一个912 bp的开放阅读框架的鉴定。后者编码一种由304个氨基酸组成的新型34 kDa接头组蛋白，暂命名为H1oo。氨基酸BLAST分析显示，H1oo显示最高的序列同源性卵母细胞特异性B4组蛋白的青蛙，各自的中央球状（推定的DNA结合）域显示54%的同一性。海胆卵母细胞的CS-H1蛋白质的实质性同源性也是显而易见的。虽然大多数对应于体细胞连接组蛋白的卵母细胞mRNA没有被多聚腺苷酸化（并且保持未翻译），但（非哺乳动物）卵母细胞特异性连接组蛋白和哺乳动物H1oo的mRNA被多聚腺苷酸化，这是由在H1oo cDNA的3 '-非翻译区中检测到的共有信号序列AAUAAA驱动的过程。我们的数据表明，小鼠卵母细胞特异性连接组蛋白H1oo（1）构成了蛙卵母细胞特异性连接组蛋白B4和海胆cs-H1连接组蛋白的一种新的哺乳动物同源物;（2）早在GV（PI）期卵母细胞表达，持续到MII期卵母细胞、卵细胞极体和二细胞胚胎，灭绝在4 - 8细胞胚胎阶段变得明显;（3）可能在卵子发生和早期胚胎发生过程中控制基因表达中起关键作用，可能是通过干扰染色质结构。
Oocytes and early embryos of multiple (non-mammalian) species lack the somatic form of the linker histone H1. To the best of our knowledge, a mammalian oocyte-specific linker (H1) histone(s) has not, as yet, been reported. We have uncovered the cDNA in question in the course of a differential screening (suppression subtractive hybridization (SSH)) project. Elucidation of the full-length sequence of this novel 1.2 kb cDNA led to the identification of a 912 bp open reading frame. The latter encoded a novel 34 kDa linker histone protein comprised of 304 amino acids, tentatively named H1oo. Amino acid BLAST analysis revealed that H1oo displayed the highest sequence homology to the oocyte-specific B4 histone of the frog, the respective central globular (putative DNA binding) domains displaying 54% identity. Substantial homology to the cs-H1 protein of the sea urchin oocyte was also apparent. While most oocytic mRNAs corresponding to somatic linker histones are not polyadenylated (and remain untranslated), the mRNAs of (non-mammalian) oocyte-specific linker histones and of mammalian H1oo, are polyadenylated, a process driven by the consensus signal sequence, AAUAAA, detected in the 3'-untranslated region of the H1oo cDNA. Our data suggest that the mouse oocyte-specific linker histone H1oo (1) constitutes a novel mammalian homolog of the oocyte-specific linker histone B4 of the frog and of the cs-H1 linker histone of the sea urchin; (2) is expressed as early as the GV (PI) stage oocyte, persisting into the MII stage oocyte, the oocytic polar bodies, and the two-cell embryo, extinction becoming apparent at the four- to eight-cell embryonic stage; and (3) may play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure.