Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement.amplification
Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement.amplification
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DOI：
10.1016/j.bios.2014.01.015
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发表时间：
2014-06-15
影响因子：
12.6
通讯作者：
Zeng, Lingwen
中科院分区：
文献类型：
--
作者：
Fang, Zhiyuan;Wu, Wei;Zeng, Lingwen
方便、灵敏的食源性病原体即时快速诊断检测一直是临床医生的需求。传统的方法，如基于培养的方法具有良好的灵敏度和特异性，但它们往往繁琐且耗时。本文提出了一种简单、灵敏的基于核酸适体的生物传感器，用于沙门氏菌的快速检测。（掌声）。其中一个适体特异性地针对S.将磁珠用于磁珠富集。另一种针对S.以大肠杆菌为报告基因，采用等温链置换扩增（SDA）技术进行扩增，并通过侧流生物传感器进行检测。低至10（1）个菌落形成单位（CFU）的S。在本研究中检测到了β-内酰胺酶。无需DNA提取，减少的操作和更简单的设备要求使该测定法成为常规方法的简单快速的替代方法。(C)2014作者由Elsevier B. V.发布。这是CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/3.0/）。
Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Traditional approaches such as culture-based methods have good sensitivity and specificity, but they tend to be tedious and time-consuming. Herein we present a simple and sensitive aptamer based biosensor for rapid detection of Salmonella enteritidis (S. enteritidis). One of the aptamers specific for the outmembrane of S. enteritidis was used for magnetic bead enrichments. Another aptamer against S. enteritidis was used as a reporter for this pathogen, which was amplified by isothermal strand displacement amplification (SDA) and further detected by a lateral flow biosensor. As low as 10(1) colony forming unit (CFU) of S. enteritidis was detected in this study. Without DNA extraction, the reduced handling and simpler equipment requirement render this assay a simple and rapid alternative to conventional methods. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).