Role of lateral cell-cell border location and extracellular/transmembrane domains in PECAM/CD31 mechanosensation
Role of lateral cell-cell border location and extracellular/transmembrane domains in PECAM/CD31 mechanosensation
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DOI：
10.1016/j.bbrc.2004.06.055
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发表时间：
2004-08-06
影响因子：
3.1
通讯作者：
Davies, PF
中科院分区：
文献类型：
--
作者：
Kaufman, DA;Albelda, SM;Davies, PF
Phosphorylation of tyrosine residues on platelet-endothelial cell adhesion molecule-1 (PECAM-1), followed by signal transduction events, has been described in endothelial cells following exposure to hyperosmotic and fluid shear stress. However, it is unclear whether PECAM-1 functions as a primary mechanosensor in this process. Utilizing a PECAM-1-null EC-like cell line, we examined the importance of cellular localization and the extracellular and transmembrane domains in PECAM-1 phosphorylation responses to mechanical stress. Tyrosine phosphorylation of PECAM-1 was stimulated in response to mechanical stress in null cells transfected either with full length PECAM-1 or with PECAM-1 mutants that do not localize to the lateral cell-cell adhesion site and that do not support homophilic binding between PECAM-1 molecules. Furthermore, null cells transfected with a construct that contains the intact cytoplasmic domain of PECAM-1 fused to the extracellular and transmembrane domains of the interleukin-2 receptor also underwent mechanical stress-induced PECAM-1 tyrosine phosphorylation. These findings suggest that mechanosensitive PECAM-1 may lie downstream of a primary mechanosensor that activates a tyrosine kinase. (C) 2004 Elsevier Inc. All rights reserved.
