The endosymbiont Wolbachia pipientis induces the expression of host antioxidant proteins in an Aedes albopictus cell line.
The endosymbiont Wolbachia pipientis induces the expression of host antioxidant proteins in an Aedes albopictus cell line.
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DOI：
10.1371/journal.pone.0002083
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发表时间：
2008-05-07
期刊：
影响因子：
3.7
通讯作者：
Harris HL
中科院分区：
文献类型：
--
作者：
Brennan LJ;Keddie BA;Braig HR;Harris HL
沃尔巴克氏体是一种专性胞内细菌，通常感染节肢动物。它们是母系遗传的，能够改变宿主的发育、性别决定和生殖。生殖操纵包括雌性化、雄性杀死、单性生殖和细胞质不相容。沃尔巴克氏体避免被宿主免疫反应破坏的机制尚不清楚。由宿主产生的抗微生物肽（AMP）和活性氧（ROS）是传统抗微生物防御的第一道防线。以前的工作表明，沃尔巴克氏体感染和诱导AMP之间没有联系。在这里，我们比较了蛋白质在自然感染沃尔巴克氏体的细胞系和通过使用抗生素治愈感染的相同细胞系中的表达。每个细胞系的蛋白质提取物进行了分析，通过二维凝胶电泳和LC/MS/MS。我们的研究结果表明，上调的主机抗氧化蛋白，这是积极对抗ROS产生的有氧细胞代谢和免疫反应过程中。此外，流式细胞仪和显微镜分析表明，活性氧的生产是显着更大的沃尔巴克氏体感染的蚊子细胞，并与位于宿主细胞质中的内共生体含有空泡。这是支持沃尔巴克氏体和昆虫抗氧化系统之间的关联的第一个经验数据。
Wolbachia are obligate intracellular bacteria which commonly infect arthropods. They are maternally inherited and capable of altering host development, sex determination, and reproduction. Reproductive manipulations include feminization, male-killing, parthenogenesis, and cytoplasmic incompatibility. The mechanism by which Wolbachia avoid destruction by the host immune response is unknown. Generation of antimicrobial peptides (AMPs) and reactive oxygen species (ROS) by the host are among the first lines of traditional antimicrobial defense. Previous work shows no link between a Wolbachia infection and the induction of AMPs. Here we compare the expression of protein in a cell line naturally infected with Wolbachia and an identical cell line cured of the infection through the use of antibiotics. Protein extracts of each cell line were analyzed by two dimensional gel electrophoresis and LC/MS/MS. Our results show the upregulation of host antioxidant proteins, which are active against ROS generated by aerobic cell metabolism and during an immune response. Furthermore, flow cytometric and microscopic analysis demonstrates that ROS production is significantly greater in Wolbachia-infected mosquito cells and is associated with endosymbiont-containing vacuoles located in the host cell cytoplasm. This is the first empirical data supporting an association between Wolbachia and the insect antioxidant system.
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影响因子：
3.2
作者：
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影响因子：
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