Using NMR metabolomics to identify responses of an environmental estrogen in blood plasma of fish
Using NMR metabolomics to identify responses of an environmental estrogen in blood plasma of fish
复制标题
DOI：
10.1016/j.aquatox.2006.04.008
复制
发表时间：
2006-07-20
影响因子：
4.5
通讯作者：
Joakim Larsson, D. G.
中科院分区：
文献类型：
--
作者：
Samuelsson, Linda M.;Forlin, Lars;Joakim Larsson, D. G.
基于核磁共振（NMR）的代谢组学与多变量数据分析相结合，可能成为研究药物和其他化学品对水生生物环境影响的有价值的工具。为了探索这种方法在鱼类中的实用性，我们使用H-1 NMR代谢组学比较暴露于合成避孕雌激素炔雌醇（EE 2）的虹鳟鱼的血浆和血浆脂质提取物与对照鱼的血浆。暴露于10 ng/L但不暴露于0.87 ng/L EE 2的鱼的血浆代谢物谱受到影响，这与仅在高剂量组中诱导的卵黄蛋白原合成一致，如通过ELISA测量的。受影响的主要代谢产物为卵黄蛋白原、丙氨酸、磷脂和胆固醇。这种发现驱动的方法确定的反应可以放在背景下与以前的知识，雌激素对鱼类的影响。这增加了使用NMR代谢组学来识别药物和其他污染物的环境影响的方法的信心。(c)2006 Elsevier B. V.保留所有权利。
Nuclear magnetic resonance (NMR) based metabolomics in combination with multivariate data analysis may become valuable tools to study environmental effects of pharmaceuticals and other chemicals in aquatic organisms. To explore the usefulness of this approach in fish, we have used H-1 NMR metabolomics to compare blood plasma and plasma lipid extracts from rainbow trout exposed to the synthetic contraceptive estrogen ethinylestradiol (EE2) with plasma from control fish. The plasma metabolite profile was affected in fish exposed to 10 ng/L but not 0.87 ng/L of EE2, which was in agreement with an induced vitellogenin synthesis in the high dose group only, as measured by ELISA. The main affected metabolites were vitellogenin, alanine, phospholipids and cholesterol. The responses identified by this discovery-driven method could be put in context with previous knowledge of the effects of estrogens on fish. This adds confidence to the approach of using NMR metabolomics to identify environmental effects of pharmaceuticals and other contaminants. (c) 2006 Elsevier B.V. All rights reserved.