Hypergravity modifies the signal transduction of ionizing radiation through p53.
Hypergravity modifies the signal transduction of ionizing radiation through p53.
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超重力通过 p53 改变电离辐射的信号转导。
DOI：
10.1269/jrr.43.s261
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发表时间：
2002
影响因子：
2
通讯作者：
Yutaka Okumura
中科院分区：
文献类型：
--
作者：
K. Okaichi;Aya Usui;Takeo Ohnishi;Yutaka Okumura
为了确定超重力对电离辐射信号转导的可能影响，我们使用蛋白质印迹分析法分析了p53的积累和p53依赖基因Waf-1和Bax的表达。超重力（20 x g）在孵育3小时后诱导人胶质母细胞瘤细胞系A172中p53的积累。低剂量（0.5戈伊）照射1.5 h后，细胞内p53表达增加，并诱导Waf-1和Bax表达。在超重（20 x g）条件下，p53的积累高峰由照射后1.5 h移至3 h，Waf-1和Bax的诱导作用完全被抑制。这些结果表明，超重力通过细胞中的p53改变了电离辐射的信号转导。
To determine the possible effect of hypergravity to modify the signal transduction of ionizing radiation, we analyzed the accumulation of p53 and the expression of p53-dependent genes, Waf-1 and Bax, using the western blot analysis. Hypergravity (20 x g) induced the accumulation of p53 in the human glioblastoma cell line A172 after 3 h of incubation. Low-dose (0.5 Gy) irradiation to the cells accumulated p53 1.5 h after irradiation, and induced Waf-1 and Bax. Under the condition of hypergravity (20 x g), the peak of p53 accumulation was shifted from 1.5 h to 3 h after irradiation, and the inductions of Waf-1 and Bax were suppressed entirely. These results indicate that hypergravity modifies the signal transduction of ionizing radiation through p53 in the cells.
DOI：
10.1073/pnas.96.24.13777
发表时间：
1999-11-23
影响因子：
11.1
作者：
Chehab, NH;Malikzay, A;Halazonetis, TD
通讯作者：
Halazonetis, TD