Prognostic value of the CD4+/CD8+ ratio of tumour infiltrating lymphocytes in colorectal cancer and HLA-DR expression on tumour cells
Prognostic value of the CD4+/CD8+ ratio of tumour infiltrating lymphocytes in colorectal cancer and HLA-DR expression on tumour cells
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DOI：
10.1007/s00262-003-0388-5
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发表时间：
2003-07-01
影响因子：
5.8
通讯作者：
Fenger, C
中科院分区：
文献类型：
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作者：
Diederichsen, ACP;Hjelmborg, JV;Fenger, C
本研究旨在探讨大肠癌细胞HLA-DR表达及肿瘤浸润淋巴细胞CD 4（+）/CD 8（+）比值与大肠癌预后的关系。应用流式细胞术，我们研究了70例酶分离的结直肠癌中肿瘤细胞的HLA II类表达和41例肿瘤浸润淋巴细胞（TILs）的表型。肿瘤细胞上HLA-DR表达的三个水平（低、中、高）之间的5年生存率没有趋势。CD 4（+）/CD 8（+）比值低的患者临床病程较好，5年生存率显著较高，p=0.046，与Dukes分期和年龄无关。我们的研究结果对肿瘤免疫学有影响;结直肠癌细胞可能是细胞毒性T淋巴细胞的靶点，但肿瘤细胞不能启动免疫反应。免疫系统的刺激可以使用体外培养的树突状细胞并加载肿瘤抗原来获得。
The purpose of this study was to clarify whether HLA-DR expression of colorectal tumour cells or the CD4(+)/CD8(+) ratio of the tumour infiltrating lymphocytes is significantly associated with the prognosis of colorectal cancer. Using flow cytometry, we studied the tumour cell expression of the HLA class II in 70 enzymatically dissociated colorectal cancers and the phenotype of tumour infiltrating lymphocytes (TILs) in 41 cases. There was no trend in 5-year survival between three levels (low, medium, high) of HLA-DR expression on the tumour cells. Patients with low CD4(+)/CD8(+) ratios had a better clinical course, with significantly higher 5-year survival, p=0.046, independent of the Dukes stage and age. Our results have implications for tumour immunology; colorectal cancer cells might be a target for cytotoxic T-lymphocytes, however the tumour cells are not able to initiate an immune response. Stimulation of the immune system could possible be obtained using dendritic cells cultured in vitro and loaded with tumour antigens.