Identification of pathogenic bacteria in blood cultures: Comparison between conventional and PCR methods
Identification of pathogenic bacteria in blood cultures: Comparison between conventional and PCR methods
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DOI：
10.1016/j.mimet.2009.07.008
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发表时间：
2009-09-01
影响因子：
2.2
通讯作者：
Lazarovitch, Tsilia
中科院分区：
文献类型：
--
作者：
Pechorsky, Alina;Nitzan, Yeshayahu;Lazarovitch, Tsilia
在一家医疗中心的一项调查中，金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌被发现是最常见的引起菌血症的细菌。一种用于鉴定血液培养中这五种最常见病原体的聚合酶链反应（PCR）方法被研发出来。为每种病原体选择了一个独特的序列并用于引物设计。61份（来自住院患者的）检测到有细菌生长的血液样本通过传统微生物学方法和PCR方法同时进行处理。PCR方法获得的结果在93.4%的病例中与传统方法获得的结果相同。PCR未能鉴定出细菌的情况仅在6.6%的病例中出现（这些病例中大多数是PCR试剂盒未涵盖的细菌）。另一组来自患者的88份血液样本在送达实验室后立即进行处理，取等份用于PCR方法。血液样本瓶同时通过传统方法进行处理。在78.4%的病例中，两种方法的结果相同。在12.5%的病例中，PCR能够鉴定出细菌，但传统方法显示样本中无细菌。另一方面，PCR得出9.1%的阴性结果，而传统方法鉴定出PCR试剂盒未涵盖的细菌。结论是，分子方法似乎是一种用于鉴定血液样本中致病细菌的特异性和精确性的方法。（C）2009爱思唯尔有限公司。保留所有权利。
Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Acinetobacter baumanii, and Klebsiella pneumoniae were found to be the most prevalent bacteremia-causing bacteria in a survey in a medical center. A PCR method for identification of these five most common pathogens in blood cultures was developed. A unique sequence was chosen for each pathogen and used for primer design. Sixty-one blood samples (from hospitalized patients) in which bacterial growth was detected were processed in parallel by conventional microbiological methods and by the PCR method. The results obtained by PCR were identical to those obtained by conventional methods in 93.4% of the cases. PCR failed to identify bacteria which were found conventionally in only 6.6% of the cases (mostly bacteria not included in the PCR cassette). Another group of eighty-eight blood samples from patients were processed immediately upon their arrival at the laboratory by taking aliquots for the PCR method. The blood sample bottles were processed in parallel by conventional methods. In 78.4% of the cases the results of both methods were identical. In 12.5% of the cases, PCR afforded identification of bacteria but conventional methods showed no bacteria in the sample. On the other hand, PCR afforded 9.1% negative results while conventional methods identified bacteria not included in the PCR cassette. It is concluded that the molecular method appears to be a specific and precise method for identifying pathogenic bacteria in blood samples. (C) 2009 Elsevier B.V. All rights reserved.